Papel del complejo Ccr4-Not en la respuesta a estrés mediada por la MAPK Spc1 en Schizosacchamomyces pombe

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dc.contributor.advisor Rodríguez Gabriel, Miguel Ángel (dir.) Portantier, Marina
dc.contributor.other UAM. Departamento de Biología Molecular es_ES
dc.contributor.other Centro de Biología Molecular Severo Ochoa es_ES 2014-05-06T14:48:29Z 2014-05-06T14:48:29Z 2013-10-25
dc.description Tesis doctoral inédita. Universidad Autónoma de Madrid, Facultad de Ciencias, Departamento de Biología Molecular. Fecha de lectura: 25-10-2013 es_ES
dc.description.abstract MAPK pathways are the principal connection between external stimuli and internal responses in all eukaryotic cells. The Spc1 MAPK pathway, p38 ortholog in mammals, plays an important role in the response to stress in Schizosaccaromyces pombe. This pathway has been well studied in fission yeast but there are still open question about its regulation and especially how the pathway achieves a specific response to a high variety of stimuli. Upon oxidative stress, the MAPK Spc1 and the RNA binding protein Csx1 present a coordinated response. Looking for candidates that interact with Spc1 and Csx1, we observe that they interact with components of the Ccr4-Not complex in TAP purification assays. This complex has gained importance in the last years because it is thought to be a global regulator of gene expression and it is conserved along all eukaryotes. Most of the studies are from S. cerevisiae and higher eukaryotes and little is known in S. pombe. In S. cerevisiae, Ccr4-Not complex regulates processes like mRNA synthesis, mRNA degradation and protein degradation and interacts with other important cell complexes like SAGA, RNA PolII, exosome and the proteasome. It is formed by nine core subunits, a Ccr4 group (Ccr4, Caf1/Pop2) involved in deadenylation and a Not group (Not1 (scaffold), Not2, Not3, Not4/Mot2) involved in transcripcional regulation and protein modification, and the RNA binding protein Caf40/Rcd1. Other proteins seem to be part of the complex like Caf4, Caf16, Btf3 and Dhh1/Ste13. We focus our work in studying the functional and structural link between Ccr4-Not complex and Spc1 MAPK and the Ccr4-Not complex regulation of the Spc1-dependent stress response. Spc1 associates with the complex through Not1 and the deadenilases Caf1 and Ccr4. Most of the components of the complex are important for the stress response like osmotic and oxidative stress and are necessary for the response to nitrogen starvation. We show that the complex regulates the expression of Spc1- dependent stress genes and that the mechanism seems to be principally at the level of transcription elongation. We speculate a model in which Ccr4-Not complex recruits Spc1 functioning as elongator factors in response to stress. en_US
dc.format.extent 159 pag. es_ES
dc.format.mimetype application/pdf es_ES
dc.language.iso spa es_ES
dc.subject.other Saccharomyces - Tesis doctorales es_ES
dc.subject.other Proteínas quinasas - Tesis doctorales es_ES
dc.subject.other Eucariotas - Tesis doctorales es_ES
dc.title Papel del complejo Ccr4-Not en la respuesta a estrés mediada por la MAPK Spc1 en Schizosacchamomyces pombe es_ES
dc.type doctoralThesis es_ES
dc.subject.eciencia Biología y Biomedicina / Biología es_ES Reconocimiento – NoComercial – SinObraDerivada es_ES
dc.rights.accessRights openAccess
dc.authorUAM Portantier , Marina (264432)

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