Mañana, JUEVES, 24 DE ABRIL, el sistema se apagará debido a tareas habituales de mantenimiento a partir de las 9 de la mañana. Lamentamos las molestias.
Porous silicon biomaterials: PSi/Cyclodextrin drug delivery hybrids and PSi/Calcium phosphate bioceramic cell scaffolds
Author
Hernández-Montelongo, JacoboEntity
UAM. Departamento de Física AplicadaDate
2013-11-14Subjects
Material biomédico - Tesis doctorales; FísicaNote
Tesis doctoral inédita, leída en Universidad Autónoma de Madrid, Facultad de Ciencias, Departamento de Física Aplicada. Fecha de lectura: 14-11-2013Esta obra está bajo una licencia de Creative Commons Reconocimiento-NoComercial-SinObraDerivada 4.0 Internacional.
Abstract
Porous silicon (PSi) is an excellent biomaterial given its biocompatibility,
biodegradability and bioresorbability. Nevertheless, it is necessary to adapt its properties
depending on the specific application. In that sense, two new PSi-based biomaterials were
developed to work in the field of drug delivery and cell scaffolds.
PSi-biopolymer hybrids are attractive as drug delivery devices because they
improve control over drug release kinetics and stability. According to this, two kinds of
PSi, nano- and macro-, were modified by β-cyclodextrin–citric-acid in-situ
polymerization. Both hybrids were characterized by microscopy and physicochemical
techniques. Its biocompatibility was proved by means of L132 cells in vitro culture.
Finally, both hybrids were tested as drug delivery systems with ciprofloxacin-base
(antibiotic) and prednisolone (anti-inflammatory), in two mediums, distilled water and
PBS. Results showed that both kinds of PSi/cyclodextrin hybrids, are functional for drug
delivery applications.
For the use PSi as cell scaffold in bone tissue engineering, deposition of calcium
phosphate (CaP) in its hydroxyapatite (HAP) phase provides an added value. Within this
context, the synthesis of CaP/PSi composites by means of two different techniques was
developed: Cyclic Spin Coating (CSC) and Cyclic Electrochemical Activation (CEA).
CSC and CEA consisted on alternate Ca and P deposition steps on PSi. Each technique
produced specific morphologies and CaP phases using the same independent Ca and P
stem-solutions at neutral pH. The brushite (BRU) phase was favored with CSC and the
HAP phase was better synthesized using CEA. Analyses by RBS on CaP/PSi synthesized
by CEA supported that, by controlling the CEA parameters, an HAP coating can be
promoted. Besides, its PSi-CaP interface was studied by HAXPES. Biocompatibility was
evaluated by bone-derived progenitor cells. Depending on the composite used to culture
cells, a characteristic response of adhered cells was obtained. These results can be used
for the design and optimization of CaP/PSi composites with enhanced biocompatibility
for bone-tissue engineering.
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