The Trypanosoma cruzi satellite DNA OligoC-TesT and Trypanosoma cruzi kinetoplast DNA OligoC-TesT for diagnosis of Chagas disease: A multi-cohort comparative evaluation studys
Author
De Winne, Koen; Büscher, Philippe; Luquetti, Alejandro O.; Tavares, Suelene B. N.; Oliveira, Rodrigo A.; Solari, Aldo; Zulantay, Inés; Apt, Werner; Diosque, Patricio; Monje Rumi, Mercedes; Gironés Pujol, Nuria; Fresno Escudero, Manuel; López-Vélez, Rogelio; Pérez-Molina, José A.; Monge-Maillo, Begoña; García, Lineth; Deborggraeve, StijnEntity
UAM. Departamento de Biología MolecularPublisher
Public Library of ScienceDate
2014-01-01Citation
10.1371/journal.pntd.0002633
PLOS Neglected Tropical Diseases 8.1 (2014): e2633
ISSN
1935-2727 (print); 1935-2735 (online)DOI
10.1371/journal.pntd.0002633Funded by
This work was financially supported by the European Commission Seventh Framework Programme, ChagasEpiNet project, grant number 223034.Project
Comunidad de Madrid. S2010/BMD-2332/INDISNET; info:eu-repo/grantAgreement/EC/FP7/223034Editor's Version
http://dx.doi.org/10.1371/journal.pntd.0002633Subjects
Combinatorial code; Drosophila; FMRFa; Neuropeptidergic cell identity; Temporal genes; Terminal differentiation; Biología y Biomedicina / BiologíaRights
© 2014 De Winne et al.Abstract
Background: The Trypanosoma cruzi satellite DNA (satDNA) OligoC-TesT is a standardised PCR format for diagnosis of
Chagas disease. The sensitivity of the test is lower for discrete typing unit (DTU) TcI than for TcII-VI and the test has not been
evaluated in chronic Chagas disease patients.
Methodology/Principal Findings: We developed a new prototype of the OligoC-TesT based on kinetoplast DNA (kDNA)
detection. We evaluated the satDNA and kDNA OligoC-TesTs in a multi-cohort study with 187 chronic Chagas patients and
88 healthy endemic controls recruited in Argentina, Chile and Spain and 26 diseased non-endemic controls from D.R. Congo
and Sudan. All specimens were tested in duplicate. The overall specificity in the controls was 99.1% (95% CI 95.2%–99.8%)
for the satDNA OligoC-TesT and 97.4% (95% CI 92.6%–99.1%) for the kDNA OligoC-TesT. The overall sensitivity in the
patients was 67.9% (95% CI 60.9%–74.2%) for the satDNA OligoC-TesT and 79.1% (95% CI 72.8%–84.4%) for the kDNA
OligoC-Test.
Conclusions/Significance: Specificities of the two T. cruzi OligoC-TesT prototypes are high on non-endemic and endemic
controls. Sensitivities are moderate but significantly (p = 0.0004) higher for the kDNA OligoC-TesT compared to the satDNA
OligoC-TesT.
Files in this item
Google Scholar:De Winne, Koen
-
Büscher, Philippe
-
Luquetti, Alejandro O.
-
Tavares, Suelene B. N.
-
Oliveira, Rodrigo A.
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Solari, Aldo
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Zulantay, Inés
-
Apt, Werner
-
Diosque, Patricio
-
Monje Rumi, Mercedes
-
Gironés Pujol, Nuria
-
Fresno Escudero, Manuel
-
López-Vélez, Rogelio
-
Pérez-Molina, José A.
-
Monge-Maillo, Begoña
-
García, Lineth
-
Deborggraeve, Stijn
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