Mañana, JUEVES, 24 DE ABRIL, el sistema se apagará debido a tareas habituales de mantenimiento a partir de las 9 de la mañana. Lamentamos las molestias.
A critical appraisal of quantitative studies of protein degradation in the framework of cellular proteostasis
Entity
UAM. Departamento de Bioquímica; UAM. Departamento de Matemáticas; Instituto de Investigaciones Biomédicas "Alberto Sols" (IIBM)Publisher
Hindawi Publishing CorporationDate
2012-11-19Citation
10.1155/2012/823597
Biochemistry Research International 2012 (2012): 823597
ISSN
2090-2247 (print); 2090-2255 (online)DOI
10.1155/2012/823597Funded by
This work was supported by Grants from MINECO SAF-2011-22643, Comunidad de Madrid P2010_BMD-2331Project
Comunidad de Madrid. S2010/BMD-2331/NEURODEGMODELSEditor's Version
http://dx.doi.org/10.1155/2012/823597Subjects
Protein degradation; Cellular proteostasis; Biología y Biomedicina / BiologíaRights
Copyright © 2012 Beatriz Alvarez-Castelao et al.Abstract
Protein homeostasis, proteostasis, is essential to understand cell function. Protein degradation is a crucial component of the proteostatic
mechanisms of the cell. Experiments on protein degradation are nowadays present in many investigations in the field of
molecular and cell biology. In the present paper, we focus on the different experimental approaches to study protein degradation
and present a critical appraisal of the results derived from steady-state and kinetic experiments using detection of unlabelled and
labelled proteinmethodologies with a proteostatic perspective. This perspective allows pinpointing the limitations in interpretation
of results and the need of further experiments and/or controls to establish “definitive evidence” for the role of protein degradation
in the proteostasis of a given protein or the entire proteome. We also provide a spreadsheet for simple calculations of mRNA and
protein decays for mimicking different experimental conditions and a checklist for the analysis of experiments dealing with protein
degradation studies that may be useful for researchers interested in the area of protein turnover.
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Google Scholar:Alvarez-Castelao, Beatriz
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Ruiz-Rivas Hernando, Carmen
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Castaño, José G.
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