Sensitive and specific serodiagnosis of Leishmania infantum infection in dogs by using peptides selected from hypothetical proteins identified by an immunoproteomic approach
Author
Chávez-Fumagalli, Miguel Angel; Martins, Vivian Tamietti; Testasicca, Miriam C S; Lage, Daniela P.; Costa, Lourena Emanuele; Lage, Paula Sousa; Duarte, Mariana Costa; Ker, Henrique Gama; Ribeiro, Tatiana Gomes; Carvalho, Fernando Aécio A; Régis, Wiliam C B; Dos Reis, Alexandre B.; Tavares, Carlos Alberto Pereira; Soto Álvarez, Manuel; Fernandes, Ana Paula Salles Moura; Coelho, Eduardo Anton̂io FerrazEntity
UAM. Departamento de Biología Molecular; Centro de Biología Molecular Severo Ochoa (CBM)Publisher
American Society for Microbiology.Date
2013-06-01Citation
10.1128/CVI.00023-13
Clinical and Vaccine Immunology 20.6 (2013): 835-841
ISSN
1556-6811 (print); 1556-679X (online)DOI
10.1128/CVI.00023-13Funded by
This work was supported by grants from the Pró-Reitoria de Pesquisa from UFMG (Edital 07/2012), Instituto Nacional de Ciência e Tecnologia em Nano-biofarmacêutica (INCT-NANOBIOFAR, Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) (CBB-APQ-02364-08, CBB-APQ-00356-10, CBB-APQ-00496-11, and CBB-APQ-00819-12), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (APQ-472090/2011-9), and the Instituto Nacional de Ciência e Tecnologia em Vacinas (INCT-V). E.A.F.C. and A.P.F. are CNPq grant recipients. M.A.C.-F. is a FAPEMIG/CAPES grant recipient. This study was supported in Spain, in part, by grants from the Ministerio de Ciencia e Innovación (FIS/PI1100095).Editor's Version
http://dx.doi.org/10.1128/CVI.00023-13Subjects
Leishmania infantum; Hypothetical proteins; Immunoproteomic approach; Biología y Biomedicina / BiologíaRights
© 2013, American Society for MicrobiologyAbstract
In Brazil, the percentage of infected dogs living in areas where canine visceral leishmaniasis (CVL) is endemic ranges from 10 to
62%; however, the prevalence of infection in dogs is probably higher than figures reported from serological studies. In addition,
problems with the occurrence of false-positive or false-negative results in the serodiagnosis of CVL have been reported. The
present work analyzed the potential of synthetic peptides mapped from hypothetical proteins for improvement of the serodiagnosis
of Leishmania infantum infection in dogs. From 26 identified leishmanial proteins, eight were selected, considering that no
homologies between these proteins and others from trypanosomatide sequence databases were encountered. The sequences of
these proteins were mapped to identify linear B-cell epitopes, and 17 peptides were synthesized and tested in enzyme-linked immunosorbent
assays (ELISAs) for the serodiagnosis of L. infantum infection in dogs. Of these, three exhibited sensitivity and
specificity values higher than 75% and 90%, respectively, to differentiate L. infantum-infected animals from Trypanosoma cruziinfected
animals and healthy animals. Soluble Leishmania antigen (SLA) showed poor sensitivity (4%) and specificity (36%) to
differentiate L. infantum-infected dogs from healthy and T. cruzi-infected dogs. Lastly, the three selected peptides were combined
in different mixtures and higher sensitivity and specificity values were obtained, even when sera from T. cruzi-infected
dogs were used. The study’s findings suggest that these three peptides can constitute a potential tool for more sensitive and specific
serodiagnosis of L. infantum infection in dogs
Files in this item
Google Scholar:Chávez-Fumagalli, Miguel Angel
-
Martins, Vivian Tamietti
-
Testasicca, Miriam C S
-
Lage, Daniela P.
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Costa, Lourena Emanuele
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Lage, Paula Sousa
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Duarte, Mariana Costa
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Ker, Henrique Gama
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Ribeiro, Tatiana Gomes
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Carvalho, Fernando Aécio A
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Régis, Wiliam C B
-
Dos Reis, Alexandre B.
-
Tavares, Carlos Alberto Pereira
-
Soto Álvarez, Manuel
-
Fernandes, Ana Paula Salles Moura
-
Coelho, Eduardo Anton̂io Ferraz
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