Microbial community composition of anoxic marine sediments in the Bay of Cádiz (Spain)

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dc.contributor.author González-Mazo, Eduardo
dc.contributor.author Sanz, José Luis
dc.contributor.author Amils, Ricardo
dc.contributor.author Köchling, Thorsten
dc.contributor.author Lara-Martín, Pablo
dc.contributor.other UAM. Departamento de Biología Molecular es_ES
dc.date.accessioned 2015-02-23T14:08:18Z
dc.date.available 2015-02-23T14:08:18Z
dc.date.issued 2011-09-01
dc.identifier.citation International Microbiology 14. 3 (2011): 143-154 en_US
dc.identifier.issn 1139-6709 (print) es_ES
dc.identifier.issn 1618-1905 (online) es_ES
dc.identifier.uri http://hdl.handle.net/10486/663993
dc.description.abstract The composition of the microbial community inhabiting the anoxic coastal sediments of the Bay of Cádiz (southern Spain) was investigated using a molecular approach consisting of PCR cloning and denaturing gradient gel electrophoresis (DGGE), based on 16S rRNA sequences. The total cell count was 1-5 × 108 cells/g sediment and, as determined by catalyzed reporter deposition-fluorescent in situ hybridization (CARD-FISH), the proportion of Bacteria to Archaea was about 70:30. The analysis of 16S-rRNA gene sequences revealed a wide spectrum of microorganisms, which could be grouped into 111 operational taxonomic units (OTUs). Many of the OTUs showed high phylogenetic similarity to microorganisms living in marine sediments of diverse geographic origin. The phylogenetic groups that were predominantly detected were Firmicutes, Deltaproteobacteria, and Gammaproteobacteria, accounting for 23, 15, and 14% of the clones, respectively. Diversity in the domain Archaea was significantly lower than in the domain Bacteria. The majority of the archaeal OTUs belonged to the Crenarchaeota phylum. Since most of the sequences could not be identified precisely at the genus/species level, the functional roles of the microorganisms in the ecosystem could not be inferred. However, seven OTUs affiliated with the Delta- and Epsilonproteobacteria were identified down to the genus level, with all of the identified genera known to occur in sulfate-rich marine environments en_US
dc.description.sponsorship This work was partially supported by grant REN2001-2980-C02-02 from the Spanish Ministry of Education and Science to J.L.S. We express our gratitude to PETRESA for funding under contract FGUAM 011301 en_US
dc.format.extent 12 pag. es_ES
dc.format.mimetype application/pdf en
dc.language.iso eng en
dc.publisher Elsevier B.V. en_US
dc.relation.ispartof International Microbiology en_US
dc.rights © Copyright 2012 Elsevier B.V. en_US
dc.subject.other 16s rDNA gene library es_ES
dc.subject.other Anoxic marine sediments es_ES
dc.subject.other Microbial community composition es_ES
dc.title Microbial community composition of anoxic marine sediments in the Bay of Cádiz (Spain) es_ES
dc.type article es_ES
dc.subject.eciencia Biología y Biomedicina / Biología es_ES
dc.relation.publisherversion http://dx.doi.org/10.2436/20.1501.01.143 es_ES
dc.identifier.doi 10.2436/20.1501.01.143 es_ES
dc.identifier.publicationfirstpage 143 es_ES
dc.identifier.publicationissue 3 es_ES
dc.identifier.publicationlastpage 154 es_ES
dc.identifier.publicationvolume 14 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion en
dc.rights.cc Reconocimiento – NoComercial – CompartirIgual es_ES
dc.rights.accessRights openAccess es_ES

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