Mañana, JUEVES, 24 DE ABRIL, el sistema se apagará debido a tareas habituales de mantenimiento a partir de las 9 de la mañana. Lamentamos las molestias.
Breast feeding increases vasoconstriction induced by electrical field stimulation in rat mesenteric artery. Role of neuronal nitric oxide and ATP
Entity
UAM. Departamento de FilosofíaPublisher
Public Library of ScienceDate
2013-01-14Citation
10.1371/journal.pone.0053802
Plos One 8.1 (2013): e53802
ISSN
1932-6203 (online)DOI
10.1371/journal.pone.0053802Funded by
This study was supported by Ministerio de Ciencia e Innovación (SAF 2009-10374) and Red Cardiovascular del Fondo de Investigaciones Sanitarias (RD06/0014/0011)Subjects
Acetylcholine; Triphosphate; Electric Stimulation; Mesenteric Arteries; Sprague-Dawley; Vasoconstriction; MedicinaRights
© 2013 Blanco-Rivero et al.Abstract
Objectives: The aim of this study was to investigate in rat mesenteric artery whether breast feeding (BF) affects the vasomotor response induced by electrical field stimulation (EFS), participation by different innervations in the EFS-induced response and the mechanism/s underlying these possible modifications. Methods: Experiments were performed in female Sprague-Dawley rats (3 months old), divided into three groups: Control (in oestrous phase), mothers after 21 days of BF, and mothers that had recovered their oestral cycle (After BF, in oestrous phase). Vasomotor response to EFS, noradrenaline (NA) and nitric oxide (NO) donor DEA-NO were studied. Neuronal NO synthase (nNOS) and phosphorylated nNOS (P-nNOS) protein expression were analysed and NO, superoxide anion (O2 .-), NA and ATP releases were also determined. Results: EFS-induced contraction was higher in the BF group, and was recovered after BF. 1 μmol/L phentolamine decreased the response to EFS similarly in control and BF rats. NA vasoconstriction and release were similar in both experimental groups. ATP release was higher in segments from BF rats. 0.1 mmol/L L-NAME increased the response to EFS in both control and BF rats, but more so in control animals. BF decreased NO release and did not modify O2 .- production. Vasodilator response to DEA-NO was similar in both groups, while nNOS and P-nNOS expressions were decreased in segments from BF animals. Conclusion: Breast feeding increases EFS-induced contraction in mesenteric arteries, mainly through the decrease of neuronal NO release mediated by decreased nNOS and P-nNOS expression. Sympathetic function is increased through the increased ATP release in BF rats
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Google Scholar:Blanco Rivero, Javier
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Sastre, Esther
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Caracuel, Laura
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Granado García, Miriam
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Balfagón Calvo, Gloria
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