Expression of MicroRNA-15b and the glycosyltransferase GCNT3 correlates with antitumor efficacy of rosemary diterpenes in colon and pancreatic cancer

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dc.contributor.author González-Vallinas, Margarita
dc.contributor.author Molina, Susana
dc.contributor.author Vicente, Gonzalo
dc.contributor.author Zarza, Virginia
dc.contributor.author Martín-Hernández, Roberto
dc.contributor.author García-Risco, Mónica Rodriguez
dc.contributor.author Fornari, Tiziana
dc.contributor.author Reglero, Guillermo
dc.contributor.author De Molina, Ana Ramírez
dc.contributor.other UAM. Departamento de Química Física Aplicada es_ES
dc.date.accessioned 2015-05-19T09:00:43Z
dc.date.available 2015-05-19T09:00:43Z
dc.date.issued 2014-06-03
dc.identifier.citation Plos One 9.6 (2014): e98556 en_US
dc.identifier.issn 1932-6203 (online) es_ES
dc.identifier.uri http://hdl.handle.net/10486/666248
dc.description.abstract Colorectal and pancreatic cancers remain important contributors to cancer mortality burden and, therefore, new therapeutic approaches are urgently needed. Rosemary (Rosmarinus officinalis L.) extracts and its components have been reported as natural potent antiproliferative agents against cancer cells. However, to potentially apply rosemary as a complementary approach for cancer therapy, additional information regarding the most effective composition, its antitumor effect in vivo and its main molecular mediators is still needed. In this work, five carnosic acid-rich supercritical rosemary extracts with different chemical compositions have been assayed for their antitumor activity both in vivo (in nude mice) and in vitro against colon and pancreatic cancer cells. We found that the antitumor effect of carnosic acid together with carnosol was higher than the sum of their effects separately, which supports the use of the rosemary extract as a whole. In addition, gene and microRNA expression analyses have been performed to ascertain its antitumor mechanism, revealing that up-regulation of the metabolic-related gene GCNT3 and down-regulation of its potential epigenetic modulator miR-15b correlate with the antitumor effect of rosemary. Moreover, plasmatic miR-15b down-regulation was detected after in vivo treatment with rosemary. Our results support the use of carnosic acid-rich rosemary extract as a complementary approach in colon and pancreatic cancer and indicate that GCNT3 expression may be involved in its antitumor mechanism and that miR-15b might be used as a non-invasive biomarker to monitor rosemary anticancer effect en_US
dc.description.sponsorship This work was supported by the Spanish Ministry of Science and Innovation (Plan Nacional I+D+i AGL2010-21565, RyC 2008-03734; IPT-2011-1248- 060000); Comunidad de Madrid (ALIBIRD, S2009/AGR-1469); and European Union Structural Funds en_US
dc.format.extent 10 pag. en
dc.format.mimetype application/pdf en
dc.language.iso eng en
dc.publisher Public Library of Science en_US
dc.relation.ispartof Plos One en_US
dc.rights © 2014 González-Vallinas et al. es_ES
dc.subject.other Cell Line, Tumor en_US
dc.subject.other Colonic Neoplasms en_US
dc.subject.other Diterpenes en_US
dc.subject.other MicroRNAs en_US
dc.subject.other Pancreatic Neoplasms en_US
dc.subject.other Rosmarinus en_US
dc.title Expression of MicroRNA-15b and the glycosyltransferase GCNT3 correlates with antitumor efficacy of rosemary diterpenes in colon and pancreatic cancer en_US
dc.type article en
dc.subject.eciencia Biología y Biomedicina / Biología es_ES
dc.identifier.doi 10.1371/journal.pone.0098556 es_ES
dc.identifier.publicationfirstpage e98556 es_ES
dc.identifier.publicationissue 6 es_ES
dc.identifier.publicationlastpage e98556 es_ES
dc.identifier.publicationvolume 9 es_ES
dc.relation.projectID Comunidad de Madrid. S2009/AGR-1469/ALIBIRD es_ES
dc.type.version info:eu-repo/semantics/publishedVersion en
dc.rights.accessRights openAccess en
dc.authorUAM Fornari Reale, Tiziana (260676)


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