Mañana, JUEVES, 24 DE ABRIL, el sistema se apagará debido a tareas habituales de mantenimiento a partir de las 9 de la mañana. Lamentamos las molestias.
Detection of the amoeba Entamoeba gingivalis in periodontal pockets
Entity
UAM. Departamento de Biología MolecularPublisher
EDP SciencesDate
2014-01-01Citation
10.1051/parasite/2014029
Parasite 21 (2014): 30
ISSN
1252-607X (print); 1776-1042 (online)DOI
10.1051/parasite/2014029Funded by
This study was carried out thanks to the financial support of the AMIB association and the Immune Activation Laboratory, Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas, Universidad Autónoma de Madrid, Cantoblanco, Madrid, SpainProject
Comunidad de Madrid. S2010/BMD-2332/INDISNETSubjects
Amoeba; Diagnosis; Entamoeba gingivalis; Microscopy; PCR; Periodontitis; Protist; Biología y Biomedicina / BiologíaRights
© 2014 M. Bonner et al.Abstract
Periodontitis is a public health issue, being one of the most prevalent diseases worldwide. However, the aetiology of the disease is still unclear: genetics of patients cannot explain the dispersed or isolated localisation of gingival pockets, while bacteria-based models are insufficient to distinguish gingivitis and periodontitis. The possible role of parasites in the establishment of periodontitis has been poorly studied until now. The aim of this project was to study a potential link between colonisation of gingival crevices by the amoeba Entamoeba gingivalis and periodontitis. In eight different dental clinics in France, samples were taken in periodontal pockets (72) or healthy sites (33), and submitted to microscopic observation and molecular identification by PCR with a new set of primers designed to specifically detect E. gingivalis. This blind sample analysis showed the strong sensitivity of PCR compared with clinical diagnosis (58/72 = 81%), and microscopy (51/65 = 78%). The results of this work show that the parasites detected by microscopy mainly – if not exclusively – belong to the species E. gingivalis and that the presence of the parasite is correlated with periodontitis
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Google Scholar:Bonner, Mark
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Amard, Véronique
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Bar-Pinatel, Charlotte
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Charpentier, Frédéric
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Chatard, Jean-Michel
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Desmuyck, Yvan
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Ihler, Serge
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Rochet, Jean-Pierre
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Roux de La Tribouille, Véronique
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Saladin, Luc
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Verdy, Marion
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Gironés Pujol, Nuria
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Fresno Escudero, Manuel
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Santi-Rocca, Julien
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