Sumoylated hnRNPA2B1 controls the sorting of miRNAs into exosomes through binding to specific motifs

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dc.contributor.author Villarroya Beltri, Carolina
dc.contributor.author Gutiérrez-Vázquez, Cristina
dc.contributor.author Sánchez-Cabo, Fátima
dc.contributor.author Pérez-Hernández, Daniel
dc.contributor.author Vázquez, Jesús
dc.contributor.author Martín-Cófreces, Noa
dc.contributor.author Martínez-Herrera, Dannys Jorge
dc.contributor.author Pascual-Montano, Alberto
dc.contributor.author Mittelbrunn, María
dc.contributor.author Sánchez Madrid, Francisco
dc.contributor.other UAM. Departamento de Medicina es_ES
dc.date.accessioned 2015-06-03T13:01:06Z
dc.date.available 2015-06-03T13:01:06Z
dc.date.issued 2013-12-20
dc.identifier.citation Nature Communications 4 (2013): 2980 en_US
dc.identifier.issn 2041-1723 es_ES
dc.identifier.uri http://hdl.handle.net/10486/666582
dc.description.abstract Exosomes are released by most cells to the extracellular environment and are involved in cell-to-cell communication. Exosomes contain specific repertoires of mRNAs, microRNAs (miRNAs) and other non-coding RNAs that can be functionally transferred to recipient cells. However, the mechanisms that control the specific loading of RNA species into exosomes remain unknown. Here we describe sequence motifs present in miRNAs that control their localization into exosomes. The protein heterogeneous nuclear ribonucleoprotein A2B1 (hnRNPA2B1) specifically binds exosomal miRNAs through the recognition of these motifs and controls their loading into exosomes. Moreover, hnRNPA2B1 in exosomes is sumoylated, and sumoylation controls the binding of hnRNPA2B1 to miRNAs. The loading of miRNAs into exosomes can be modulated by mutagenesis of the identified motifs or changes in hnRNPA2B1 expression levels. These findings identify hnRNPA2B1 as a key player in miRNA sorting into exosomes and provide potential tools for the packaging of selected regulatory RNAs into exosomes and their use in biomedical applications en_US
dc.description.sponsorship This work was supported by grants SAF2011-25834, ERC-2011-AdG, BIO2009_07990, BIO2010-17527, COST-Action BM1202, P210/BMD-2305 and Cardiovascular Network RD12-0042-0056 from Instituto Salud Carlos III. C.V.B. was supported by FPU program (Spanish Ministry of Education). M.M. was supported by the Instituto de Salud Carlos III, C.G.-V. by the Comunidad de Madrid and D.J.M.H. by Programa La Caixa es_ES
dc.format.extent 10 pag. es_ES
dc.format.mimetype application/pdf en
dc.language.iso eng es_ES
dc.publisher Nature Publishung Group en_US
dc.relation.ispartof Nature Communications en_US
dc.rights © 2013 Macmillan Publishers Limited en_US
dc.subject.other Exosomes en_US
dc.subject.other MicroRNAs en_US
dc.subject.other Mutagenesis en_US
dc.subject.other Sumoylation en_US
dc.title Sumoylated hnRNPA2B1 controls the sorting of miRNAs into exosomes through binding to specific motifs en_US
dc.type article en
dc.subject.eciencia Medicina es_ES
dc.identifier.doi 10.1038/ncomms3980 es_ES
dc.identifier.publicationfirstpage 2980 es_ES
dc.identifier.publicationlastpage 2980 es_ES
dc.identifier.publicationvolume 4 es_ES
dc.relation.projectID Comunidad de Madrid. S2010/BMD-2332/INDISNET es_ES
dc.relation.projectID Comunidad de Madrid. S2010/BMD-2305/CS INTERACTOMICS es_ES
dc.type.version info:eu-repo/semantics/publishedVersion en
dc.rights.cc Reconocimiento – NoComercial – SinObraDerivada es_ES
dc.rights.accessRights openAccess en
dc.authorUAM Mittelbrum Herrero, María (279535)
dc.authorUAM Villarroya Beltrí, Carolina (264703)
dc.authorUAM Sánchez Madrid, Francisco (259404)


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