Degradation of IF1 controls energy metabolism during osteogenic differentiation of stem cells
Entity
UAM. Departamento de Biología MolecularPublisher
EMBO PressDate
2013-06-01Citation
10.1038/embor.2013.72
EMBO Reports 14.7 (2013): 638-644
ISSN
1469-221X (print); 1469-3178 (online)DOI
10.1038/embor.2013.72Funded by
This work was supported by grants from the Ministerio de Educación y Ciencia (BFU2010-18903), the Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER), ISCIII, and Comunidad de Madrid (S2011/BMD-2402), SpainProject
Comunidad de Madrid. S2010/BMD-2402/MITOLABSubjects
ATPase inhibitory factor 1; cellular differentiation; H+-ATP synthase; mitochondria; protein degradation; Biología y Biomedicina / BiologíaRights
© 2013 European Molecular Biology OrganizationAbstract
Differentiation of human mesenchymal stem cells (hMSCs) requires the rewiring of energy metabolism. Herein, we demonstrate that the ATPase inhibitory factor 1 (IF1) is expressed in hMSCs and in prostate and colon stem cells but is not expressed in the differentiated cells. IF1 inhibits oxidative phosphorylation and regulates the activity of aerobic glycolysis in hMSCs. Silencing of IF1 in hMSCs mimics the metabolic changes observed in osteocytes and accelerates cellular differentiation. Activation of IF1 degradation acts as the switch that regulates energy metabolism during differentiation. We conclude that IF1 is a stemness marker important for maintaining the quiescence state
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Google Scholar:Sánchez-Aragó, María
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García-Bermúdez, Javier
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Martínez-Reyes, Inmaculada
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Santacatterina, Fulvio
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Cuezva Marcos, José Manuel
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