Imbalance between pro and anti-oxidant mechanisms in perivascular adipose tissue aggravates long-term high-fat diet-derived endothelial dysfunction
Entity
UAM. Departamento de FisiologíaPublisher
Public Library of ScienceDate
2014-04-23Citation
10.1371/journal.pone.0095312
Plos One 9.4 (2014): e95312
ISSN
1932-6203 (online)DOI
10.1371/journal.pone.0095312Funded by
This work was supported by grants from Ministerio de Ciencia e Investigación (BFU2011-25303), Ministerio de Economía y Competitividad (SAF2009- 09714, SAF2011-25303, BFU2012-35353), Grupos Universidad Complutense de Madrid (UCM; GR-921641), Fundación Universitaria San Pablo-CEU, Fundación Mutua Madrileña and Sociedad para el Estudio de la Salud Cardiometabólica (SESCAMET). MGO and CFG-P are supported by Ministerio de Educación y CienciaSubjects
adipose tissue; antioxidant activity; endothelial dysfunction; enzyme activity; mesenteric artery; obesity; MedicinaRights
© 2014 Gil-Ortega et al.Abstract
Background: The hypothesis of this study is that long-term high-fat diets (HFD) induce perivascular adipose tissue (PVAT) dysfunction characterized by a redox imbalance, which might contribute to aggravate endothelial dysfunction in obesity. Methods and Results: C57BL/6J mice were fed either control or HFD (45% kcal from fat) for 32 weeks. Body weight, lumbar and mesenteric adipose tissue weights were significantly higher in HFD animals compared to controls. The anticontractile effect of PVAT in mesenteric arteries (MA) was lost after 32 week HFD and mesenteric endothelial-dependent relaxation was significantly impaired in presence of PVAT in HFD mice (Emax = 71.0±5.1 vs Emax = 58.5±4.2, p<0.001). The inhibitory effect of L-NAME on Ach-induced relaxation was less intense in the HFD group compared with controls suggesting a reduction of endothelial NO availability. Expression of eNOS and NO bioavailability were reduced in MA and almost undetectable in mesenteric PVAT of the HFD group. Superoxide levels and NOX activity were higher in PVAT of HFD mice. Apocynin only reduced contractile responses to NA in HFD animals. Expression of ec-SOD and total SOD activity were significantly reduced in PVAT of HFD mice. No changes were observed in Mn-SOD, Cu/Zn-SOD or catalase. The ratio [GSSG]/([GSH]+[GSSG]) was 2-fold higher in the mesenteric PVAT from HFD animals compared to controls. Conclusions: We suggest that the imbalance between pro-oxidant (NOX, superoxide anions, hydrogen peroxide) and antioxidant (eNOS, NO, ecSOD, GSSG) mechanisms in PVAT after long-term HFD might contribute to the aggravation of endothelial dysfunction
Files in this item
Google Scholar:Gil-Ortega, Marta
-
Condezo-Hoyos, Luis Alberto
-
García-Prieto, Concha F.
-
Arribas Rodríguez, Silvia Magdalena
-
González Enguita, María del Carmen
-
Aránguez, Isabel I.
-
Ruiz-Gayo, Mariano
-
Somoza, Beatriz
-
Fernández-Alfonso, María Soledad
This item appears in the following Collection(s)
Related items
Showing items related by title, author, creator and subject.
-
Beneficial effect of bariatric surgery on abnormal MMP-9 and AMPK activities: Potential markers of obesity-related CV risk
García-Prieto, Concha F.; Gil-Ortega, Marta; Vega-Martín, Elena; Ramiro-Cortijo, David; Martín-Ramos, Miriam; Bordiú, Elena; Sanchez-Pernaute, Andrés; Torres, Antonio; Aránguez, I.; Fernández-Alfonso, María; Rubio, Miguel A.; Somoza, Beatriz
2019-05-08