dc.contributor.author | Lorente, Elena | |
dc.contributor.author | Infantes, Susana | |
dc.contributor.author | Barnea, Eilon | |
dc.contributor.author | Beer, Ilan | |
dc.contributor.author | García, Ruth Cabrera | |
dc.contributor.author | Lasala, Fátima | |
dc.contributor.author | Jiménez, Mercedes | |
dc.contributor.author | Vilches Ruiz, Blas Carlos | |
dc.contributor.author | Lemonnier, François A. | |
dc.contributor.author | Admon, Arie | |
dc.contributor.author | López, Daniel | |
dc.contributor.other | UAM. Departamento de Medicina | es_ES |
dc.date.accessioned | 2015-07-07T11:56:13Z | |
dc.date.available | 2015-07-07T11:56:13Z | |
dc.date.issued | 2012-01-01 | |
dc.identifier.citation | Journal of Virology 86.1 (2012): 527-541 | en_US |
dc.identifier.issn | 0022-538X (print) | en_US |
dc.identifier.issn | 1098-5514 (online) | en_US |
dc.identifier.uri | http://hdl.handle.net/10486/667283 | |
dc.description.abstract | The transporter associated with antigen processing (TAP) delivers the viral proteolytic products generated by the proteasome in
the cytosol to the endoplasmic reticulum lumen that are subsequently recognized by cytotoxic T lymphocytes (CTLs). However,
several viral epitopes have been identified in TAP-deficient models. Using mass spectrometry to analyze complex human leukocyte
antigen (HLA)-bound peptide pools isolated from large numbers of TAP-deficient vaccinia virus-infected cells, we identified
11 ligands naturally presented by four different HLA-A, HLA-B, and HLA-C class I molecules. Two of these ligands were presented
by two different HLA class I alleles, and, as a result, 13 different HLA-peptide complexes were formed simultaneously in
the same vaccinia virus-infected cells. In addition to the high-affinity ligands, one low-affinity peptide restricted by each of the
HLA-A, HLA-B, and HLA-C class I molecules was identified. Both high- and low-affinity ligands generated long-term memory
CTL responses to vaccinia virus in an HLA-A2-transgenic mouse model. The processing and presentation of two vaccinia virusencoded
HLA-A2-restricted antigens took place via proteasomal and nonproteasomal pathways, which were blocked in infected
cells with chemical inhibitors specific for different subsets of metalloproteinases. These data have implications for the study of
the effectiveness of early empirical vaccination with cowpox virus against smallpox disease | en_US |
dc.description.sponsorship | This work was supported by grants to D.L. from the Programa Ramón
y Cajal, Ministerio de Ciencia e Innovación and the FIPSE Foundation
and to A.A. from the ISF 9916/05.
The funders had no role in the study design, data collection, and analysis,
decision to publish, or preparation of the manuscript.
The authors have no conflicting financial interests | es_ES |
dc.format.extent | 15 pag. | es_ES |
dc.format.mimetype | application/pdf | en |
dc.language.iso | eng | en |
dc.publisher | American Society for Microbiology | en_US |
dc.relation.ispartof | Journal of the American Society of Nephrology | en_US |
dc.rights | © 2014, American Society for Microbiology | en_US |
dc.subject.other | Animals | en_US |
dc.subject.other | Antigen-presenting cells | es_ES |
dc.subject.other | Histocompatibility antigens class I | en_US |
dc.title | Multiple viral ligands naturally presented by different class I molecules in transporter antigen processing-deficient vaccinia virus-infected cells | en_US |
dc.type | article | en |
dc.subject.eciencia | Medicina | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1128/JVI.05737-11 | es_ES |
dc.identifier.doi | 10.1128/JVI.05737-11 | es_ES |
dc.identifier.publicationfirstpage | 1315 | es_ES |
dc.identifier.publicationissue | 7 | es_ES |
dc.identifier.publicationlastpage | 1325 | es_ES |
dc.identifier.publicationvolume | 22 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | en |
dc.rights.accessRights | openAccess | en |
dc.facultadUAM | Facultad de Medicina | |