Effects of intravenous administration of allogenic bone marrow- and adipose tissue-derived mesenchymal stem cells on functional recovery and brain repair markers in experimental ischemic stroke
Entity
UAM. Departamento de MedicinaPublisher
BioMed CentralDate
2013-02-18Citation
10.1186/scrt159
Stem Cell Research & Therapy 4.1 (2013): 11
ISSN
1757-6512DOI
10.1186/scrt159Funded by
This study was supported by grants from Cellerix, FIS 060575 and PS09/ 01606 (Spanish Ministry of Science), CIDEM (Center for Innovation and Business Development) and by RENEVAS (RD07/0026/2003) (Spanish Neurovascular Network), the Carlos III Research Institute and the Ministry of Science and InnovationProject
Gobierno de España. PS09/01606Editor's Version
http://dx.doi.org/10.1186/scrt159Subjects
Allogenic MSC; Cells; Neurogenesis; MedicinaNote
The electronic version of this article is the complete one and can be found online at: http://stemcellres.com/content/4/1/11Rights
© 2013 Gutiérrez-Fernández et al.Abstract
Introduction: Stem cell therapy can promote good recovery from stroke. Several studies have demonstrated that
mesenchymal stem cells (MSC) are safe and effective. However, more information regarding appropriate cell type is
needed from animal model. This study was targeted at analyzing the effects in ischemic stroke of acute
intravenous (i.v.) administration of allogenic bone marrow- (BM-MSC) and adipose-derived-stem cells (AD-MSC) on
functional evaluation results and brain repair markers.
Methods: Allogenic MSC (2 × 106 cells) were administered intravenously 30 minutes after permanent middle
cerebral artery occlusion (pMCAO) to rats. Infarct volume and cell migration and implantation were analyzed by
magnetic resonance imaging (MRI) and immunohistochemistry. Function was evaluated by the Rogers and rotarod
tests, and cell proliferation and cell-death were also determined. Brain repair markers were analyzed by confocal
microscopy and confirmed by western blot.
Results: Compared to infarct group, function had significantly improved at 24 h and continued at 14 d after i.v.
administration of either BM-MSC or AD-MSC. No reduction in infarct volume or any migration/implantation of cells
into the damaged brain were observed. Nevertheless, cell death was reduced and cellular proliferation significantly
increased in both treatment groups with respect to the infarct group. At 14 d after MSC administration vascular
endothelial growth factor (VEGF), synaptophysin (SYP), oligodendrocyte (Olig-2) and neurofilament (NF) levels were
significantly increased while those of glial fiibrillary acid protein (GFAP) were decreased.
Conclusions: i.v. administration of allogenic MSC - whether BM-MSC or AD-MSC, in pMCAO infarct was associated
with good functional recovery, and reductions in cell death as well as increases in cellular proliferation,
neurogenesis, oligodendrogenesis, synaptogenesis and angiogenesis markers at 14 days post-infarct
Files in this item
Google Scholar:Gutiérrez-Fernández, María
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Rodríguez-Frutos, Berta
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Ramos-Cejudo, Jaime
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Teresa Vallejo-Cremades, M.
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Fuentes Gimeno, Blanca Eulalia
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Cerdán, Sebastián
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Díez Tejedor, Exuperio
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