dc.contributor.author | Ramos-Cejudo, Jaime | |
dc.contributor.author | Gutiérrez-Fernández, María | |
dc.contributor.author | Otero-Ortega, Laura | |
dc.contributor.author | Rodríguez-Frutos, Berta | |
dc.contributor.author | Fuentes Gimeno, Blanca Eulalia | |
dc.contributor.author | Vallejo-Cremades, María Teresa | |
dc.contributor.author | Hernanz, Teresa Navarro | |
dc.contributor.author | Cerdán, Sebastián | |
dc.contributor.author | Díez Tejedor, Exuperio | |
dc.contributor.other | UAM. Departamento de Medicina | es_ES |
dc.contributor.other | Instituto de Investigaciones Biomédicas "Alberto Sols" (IIBM) | es_ES |
dc.contributor.other | Instituto de Investigación Sanitaria Hospital Universitario de La Paz (IdiPAZ) | es_ES |
dc.date.accessioned | 2016-06-01T14:22:15Z | |
dc.date.available | 2016-06-01T14:22:15Z | |
dc.date.issued | 2015-01-01 | |
dc.identifier.citation | Stroke 46.1 (2015): 221-8 | es_ES |
dc.identifier.issn | 0039-2499 | es_ES |
dc.identifier.issn | 1524-4628 (on line) | es_ES |
dc.identifier.uri | http://hdl.handle.net/10486/671186 | |
dc.description.abstract | BACKGROUND AND PURPOSE:
Translational research is beginning to reveal the importance of trophic factors as a therapy for cellular brain repair. The purpose of this study was to analyze whether brain-derived neurotrophic factor (BDNF) administration could mediate oligodendrogenesis and remyelination after white matter injury in subcortical stroke.
METHODS:
Ischemia was induced in rats by injection of endothelin-1. At 24 hours, 0.4 μg/kg of BDNF or saline was intravenously administered to the treatment and control groups, respectively. Functional evaluation, MRI, and fiber tract integrity on tractography images were analyzed. Proliferation (KI-67) and white matter repair markers (A2B5, 2',3'-cyclic-nucleotide 3'-phosphodiesterase [CNPase], adenomatous polyposis coli [APC], platelet-derived growth factor receptor alpha [PDGFR-α], oligodendrocyte marker O4 [O4], oligodendrocyte transcription factor [Olig-2], and myelin basic protein [MBP]) were analyzed at 7 and 28 days.
RESULTS:
The BDNF-treated animals showed less functional deficit at 28 days after treatment than the controls (P<0.05). Although T2-MRI did not show differences in lesion size at 7 and 28 days between groups, diffusion tensor imaging tractography analysis revealed significantly better tract connectivity at 28 days in the BDNF group than in the controls (P<0.05). Increased proliferation of oligodendrocyte progenitors was observed in treated animals at 7 days (P<0.05). Finally, the levels of white matter repair markers (A2B5, CNPase, and O4 at 7 days; Olig-2 and MBP at 28 days) were higher in the BDNF group than in the controls (P<0.05).
CONCLUSIONS:
BDNF administration exerted better functional outcome, oligodendrogenesis, remyelination, and fiber connectivity than controls in rats subjected to subcortical damage in ischemic stroke | en_US |
dc.description.sponsorship | Supported by research grants PS12/01754 (P.I.: EDT), INVICTUS Spanish
Neurovascular Network RD12/0014/0006 (BRF and JRC) and Sara Borrell postdoctoral fellowship CD12/00706 (LOO) from the Research Institute Carlos III, Ministry of
Science and Innovation of Spain | es_ES |
dc.format.extent | 29 pag. | es_ES |
dc.format.mimetype | application/pdf | en |
dc.language.iso | eng | en |
dc.publisher | American Heart Association | en_US |
dc.relation.ispartof | Stroke | en_US |
dc.rights | © 2014 American Heart Association, Inc | en_US |
dc.subject.other | Brain-derived neurotrophic factor | en_US |
dc.subject.other | Endothelin-1 | en_US |
dc.subject.other | Subcortical stroke | en_US |
dc.title | Brain-derived neurotrophic factor administration mediated oligodendrocyte differentiation and myelin formation in subcortical ischemic stroke | en_US |
dc.type | article | en |
dc.subject.eciencia | Medicina | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1161/STROKEAHA.114.006692 | en_US |
dc.identifier.doi | 10.1161/STROKEAHA.114.006692 | es_ES |
dc.identifier.publicationfirstpage | 221 | es_ES |
dc.identifier.publicationissue | 1 | es_ES |
dc.identifier.publicationlastpage | 228 | es_ES |
dc.identifier.publicationvolume | 46 | es_ES |
dc.type.version | info:eu-repo/semantics/submittedVersion | en_US |
dc.rights.accessRights | openAccess | en |
dc.authorUAM | Díez Tejedor, Exuperio (258291) | |
dc.authorUAM | Fuentes Gimeno, Blanca Eulalia (262687) | |
dc.facultadUAM | Facultad de Medicina | |
dc.institutoUAM | Instituto de Investigaciones Biomédicas "Alberto Sols" (IIBM) | |
dc.institutoUAM | Instituto de Investigación Sanitaria Hospital Universitario de La Paz (IdiPAZ) | |