Long-range projection neurons of the mouse ventral tegmental area: A single-cell axon tracing analysis

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dc.contributor.author Aransay, Ana
dc.contributor.author Rodríguez-López, Claudia
dc.contributor.author García-Amado, María
dc.contributor.author Clasca, Francisco
dc.contributor.author Prensa, Lucía
dc.contributor.other UAM. Departamento de Anatomía, Histología y Neurociencia es_ES
dc.date.accessioned 2016-07-22T13:26:50Z
dc.date.available 2016-07-22T13:26:50Z
dc.date.issued 2015-05-19
dc.identifier.citation Frontiers in Neuroanatomy 9. MAY (2015): Artículo 59 en_US
dc.identifier.issn 1662-5129 es_ES
dc.identifier.uri http://hdl.handle.net/10486/672208
dc.description.abstract Pathways arising from the ventral tegmental area (VTA) release dopamine and other neurotransmitters during the expectation and achievement of reward, and are regarded as central links of the brain networks that create drive, pleasure, and addiction. While the global pattern of VTA projections is well-known, the actual axonal wiring of individual VTA neurons had never been investigated. Here, we labeled and analyzed the axons of 30 VTA single neurons by means of single-cell transfection with the Sindbis-pal-eGFP vector in mice. These observations were complemented with those obtained by labeling the axons of small populations of VTA cells with iontophoretic microdeposits of biotinylated dextran amine. In the single-cell labeling experiments, each entire axonal tree was reconstructed from serial sections, the length of terminal axonal arbors was estimated by stereology, and the dopaminergic phenotype was tested by double-labeling for tyrosine hydroxylase immunofluorescence. We observed two main, markedly different VTA cell morphologies: neurons with a single main axon targeting only forebrain structures (FPN cells), and neurons with multibranched axons targeting both the forebrain and the brainstem (F + BSPN cells). Dopaminergic phenotype was observed in FPN cells. Moreover, four “subtypes” could be distinguished among the FPN cells based on their projection targets: (1) “Mesocorticolimbic” FPN projecting to both neocortex and basal forebrain; (2) “Mesocortical” FPN innervating the neocortex almost exclusively; (3) “Mesolimbic” FPN projecting to the basal forebrain, accumbens and caudateputamen; and (4) “Mesostriatal” FPN targeting only the caudateputamen. While the F + BSPN cells were scattered within VTA, the mesolimbic neurons were abundant in the paranigral nucleus. The observed diversity in wiring architectures is consistent with the notion that different VTA cell subpopulations modulate the activity of specific sets of prosencephalic and brainstem structures en_US
dc.description.sponsorship The project was supported by Grants from the Fundación Eugenio Rodríguez Pascual, the Spanish MINECO (BFU2010-19695) and the European Union Seventh Framework Programme (FP7/2007-2013) under grant agreement no. 604102 (Human Brain Project) en_US
dc.format.extent 24 pag. es_ES
dc.format.mimetype application/pdf en
dc.language.iso eng en
dc.publisher Frontiers Media en_US
dc.relation.ispartof Frontiers in Neuroanatomy en_US
dc.rights ©2015 Aransay, Rodríguez-López, García-Amado, Clascá and Prensa es_ES
dc.subject.other Axonal branching en_US
dc.subject.other Dopamine en_US
dc.subject.other Ventral pallidum en_US
dc.subject.other Single-cell labeling en_US
dc.subject.other Cortex en_US
dc.subject.other Thalamus en_US
dc.subject.other Parabraquial pigmented nucleus es_ES
dc.subject.other Rostral ventral tegmental area en_US
dc.title Long-range projection neurons of the mouse ventral tegmental area: A single-cell axon tracing analysis en_US
dc.type article en
dc.subject.eciencia Medicina es_ES
dc.relation.publisherversion http://dx.doi.org/10.3389/fnana.2015.00059 es_ES
dc.identifier.doi 10.3389/fnana.2015.00059 es_ES
dc.identifier.publicationfirstpage Artículo 59 es_ES
dc.identifier.publicationissue MAY en
dc.identifier.publicationlastpage Artículo 59 es_ES
dc.identifier.publicationvolume 9 es_ES
dc.relation.projectID Gobierno de España. BFU2010-19695 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/604102 en_US
dc.type.version info:eu-repo/semantics/publishedVersion en_US
dc.rights.cc Reconocimiento es_ES
dc.rights.accessRights openAccess en
dc.authorUAM Clasca Cabre, Francisco (259664)
dc.authorUAM García-Amado Sancho, María (261905)

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