Mañana, JUEVES, 24 DE ABRIL, el sistema se apagará debido a tareas habituales de mantenimiento a partir de las 9 de la mañana. Lamentamos las molestias.
Targeted gold-coated iron oxide nanoparticles for CD163 detection in atherosclerosis by MRI
Entity
UAM. Departamento de Medicina; Instituto de Investigación Sanitaria Fundación Jiménez Díaz (IIS-FJD)Publisher
Nature Publishing GroupDate
2015-11-30Citation
10.1038/srep17135
Scientific Reports 5 (2015): 1713517135
ISSN
2045-2322DOI
10.1038/srep17135Funded by
This work was funded by Spanish Government through a Plan Nacional (CTQ2011–27268), FEDER funds through the Fondo de Investigación Sanitaria (PI10/00072, PI13/00051, PI13/00395, PI13/00802, PI14/00883 and PI14/00386), CIBERDEM group, RETICS RD12/0042/0038, Programa Miguel Servet (CP10/00479) and cvREMOD CENIT project (CEN-20091044), the Basque Government through Etortek 2011 (IE11–301), and Fundacion Lilly, Spanish Society of Atherosclerosis, Spanish Society of Nephrology and Fundacion Renal Iñigo Alvarez de ToledoProject
Gobierno de España. CTQ2011–27268Editor's Version
http://dx.doi.org/10.1038/srep17135Subjects
Atherosclerosis; CD163; Sensitive targeted; Information; Atheromatous lesions; MedicinaAbstract
CD163 is a membrane receptor expressed by macrophage lineage. Studies performed in
atherosclerosis have shown that CD163 expression is increased at inflammatory sites, pointing at
the presence of intraplaque hemorrhagic sites or asymptomatic plaques. Hence, imaging of CD163
expressing macrophages is an interesting strategy in order to detect atherosclerotic plaques. We
have prepared a targeted probe based on gold-coated iron oxide nanoparticles vectorized with
an anti-CD163 antibody for the specific detection of CD163 by MRI. Firstly, the specificity of the
targeted probe was validated in vitro by incubation of the probe with CD163(+) or (−) macrophages.
The probe was able to selectively detect CD163(+) macrophages both in human and murine cells.
Subsequently, the targeted probe was injected in 16 weeks old apoE deficient mice developing
atherosclerotic lesions and the pararenal abdominal aorta was imaged by MRI. The accumulation
of probe in the site of interest increased over time and the signal intensity decreased significantly
48 hours after the injection. Hence, we have developed a highly sensitive targeted probe capable of
detecting CD163-expressing macrophages that could provide useful information about the state of
the atheromatous lesions
Files in this item
Google Scholar:Tarin, Carlos
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Carril, Monica
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Martín Ventura, José Luis
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Markuerkiaga, Irati
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Padro, Daniel
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Llamas-Granda, Patricia
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Moreno, Juan Antonio
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García, Isabel
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Genicio, Nuria
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Plaza-Garcia, Sandra
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Blanco-Colio, Luis Miguel
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Penades, Soledad
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Egido, Jesus
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