Transposase interaction with the β sliding clamp: Effects on insertion sequence proliferation and transposition rate

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dc.contributor.author Díaz-Maldonado, Héctor
dc.contributor.author Gómez, Manuel J.
dc.contributor.author Moreno-Paz, Mercedes
dc.contributor.author San Martín-Úriz, Patxi
dc.contributor.author Amils, Ricardo
dc.contributor.author Parro, Víctor
dc.contributor.author López de Saro, Francisco J.
dc.contributor.other UAM. Departamento de Biología Molecular es_ES
dc.date.accessioned 2016-08-29T10:58:40Z
dc.date.available 2016-08-29T10:58:40Z
dc.date.issued 2015-08-26
dc.identifier.citation Scientific Reports 5 (2015): 13329 en_US
dc.identifier.issn 2045-2322 es_ES
dc.identifier.uri http://hdl.handle.net/10486/672486
dc.description.abstract Insertion sequences (ISs) are ubiquitous and abundant mobile genetic elements in prokaryotic genomes. ISs often encode only one protein, the transposase, which catalyzes their transposition. Recent studies have shown that transposases of many different IS families interact with the β sliding clamp, a DNA replication factor of the host. However, it was unclear to what extent this interaction limits or favors the ability of ISs to colonize a chromosome from a phylogenetically-distant organism, or if the strength of this interaction affects the transposition rate. Here we describe the proliferation of a member of the IS1634 family in Acidiphilium over ~600 generations of cultured growth. We demonstrate that the purified transposase binds to the β sliding clamp of Acidiphilium, Leptospirillum and E. coli. Further, we also demonstrate that the Acidiphilium IS1634 transposase binds to the archaeal sliding clamp (PCNA) from Methanosarcina, and that the transposase encoded by Methanosarcina IS1634 binds to Acidiphilium β. Finally, we demonstrate that increasing the strength of the interaction between β and transposase results in a higher transposition rate in vivo. Our results suggest that the interaction could determine the potential of ISs to be mobilized in bacterial populations and also their ability to proliferate within chromosomes en_US
dc.description.sponsorship This work was funded by the Subdirección General de Proyectos de Investigación of the Spanish Ministry for Economy and Competitiveness Grants No. CGL2010-17384 and AYA2011-24803, and by the European Research Council (ERC) Advanced Grant No. 250350 (IPBSL). HDM has a FPI fellowship from the Spanish Government en_US
dc.format.extent 13 pag. en
dc.format.mimetype application/pdf en
dc.language.iso eng en
dc.publisher Nature Publishing Group en_US
dc.relation.ispartof Scientific Reports en_US
dc.subject.other Insertion sequences en_US
dc.subject.other Transposase en_US
dc.subject.other Bacterial genomes en_US
dc.subject.other Potential of ISs en_US
dc.subject.other Chromosomes. en_US
dc.title Transposase interaction with the β sliding clamp: Effects on insertion sequence proliferation and transposition rate en_US
dc.type article en
dc.subject.eciencia Biología y Biomedicina / Biología es_ES
dc.relation.publisherversion http://dx.doi.org/10.1038/srep13329 es_ES
dc.identifier.doi 10.1038/srep13329 es_ES
dc.identifier.publicationfirstpage 13329-1 es_ES
dc.identifier.publicationlastpage 13329-13 es_ES
dc.identifier.publicationvolume 5 es_ES
dc.relation.projectID Gobierno de España. CGL2010-17384 es_ES
dc.relation.projectID Gobierno de España. AYA2011-24803 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/250350
dc.type.version info:eu-repo/semantics/publishedVersion en
dc.rights.cc Reconocimiento – NoComercial – SinObraDerivada es_ES
dc.rights.accessRights openAccess en


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