Mañana, JUEVES, 24 DE ABRIL, el sistema se apagará debido a tareas habituales de mantenimiento a partir de las 9 de la mañana. Lamentamos las molestias.
Caracterización funcional y estructural de las secuencias en cis y factores en trans presentes en la región LCR del gen de la tirosinasa de ratón
Author
Regales Álvarez, LucíaAdvisor
Lluís Montoliu, JoséEntity
UAM. Departamento de Biología MolecularDate
2005-03-15Subjects
Tirosina - Tesis doctorales; Biología y Biomedicina / BiologíaNote
Tesis doctoral inédita leída en la Universidad Autónoma de Madrid, Facultad de Ciencias, Departamento de Biología Molecular. Fecha de lectura: 15-03-2005Abstract
Tyrosinase, the ratdimiting enzyrne in the melanin synthesis pathway,
constitutes an interesting model to study the mechanisrns by which expression
domains work.
The expression studies on the mouse tyrosinase gene regulation are an example
of a representative mammalian expresslon domain, regarding ik organization
within the nuclear chromatin, the establishment of its specific pattem of
expression during development and throughout the life of an organlsm. Recently,
functional dissection of regulatory elements present within this LCR have revealed
the existente of different regulatory activities, including a novel boundary
eiement.
In this report, we have studied the relevant cis sequences, involved in the
insulation of the tyrosinase gene and the hns acting factors binding to #ose
within the mouse tyrosinase LCR. We have found several DNA-protein complexes
binding to the LCR. We have identified one of them, binding to a G rich sequence,
the CTCF factor, which has been previously described as an insulator protein.
Taking advantage of the complexion of the human genome, and the comparative
analysis, we have found a new 5'upstream regulatory sequence in the human
tyrosinase gene, by comparison of the mouse LCR, which is active functionally.
On the other hand, we have ohserved the chromatin status for the LCR region,
founding a methylation and acetylation pattern which suggest a transition
between open and close status of the chromatin within tyrosinase domain.
Finally, we have generated two new genomic tools to further investigate the role
of the LCR region at the endogenous location. ñrst, a v e r designed for
homologous recombination in ES cell, and cecond, a transgene expressing the
inducible Cre recombinase under the control of tyrosinase regulatory regions.
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