Thermal Scanning at the Cellular Level by an Optically Trapped Upconverting Fluorescent Particle
Entity
UAM. Departamento de Física de MaterialesPublisher
Wiley-VCH VerlagDate
2016-01-28Citation
10.1002/adma.201505020
Advanced Materials 28.12 (2016): 2421–2426
ISSN
0935-9648 (print); 1521-4095 (online)DOI
10.1002/adma.201505020Funded by
This work was supported by the Spanish Ministerio de Educación y Ciencia (MAT2013–47395-C4–1-R) and by Banco Santander for “Proyectos de Cooperación Interuniversitaria” (2015/ASIA/06). P.H.G thanks the Spanish Ministerio de Economía y Competitividad (MINECO) for the Juan de la Cierva program. P.R.S thanks the Spanish Ministerio de Economía y Competitividad (MINECO) for the “Promoción del talento y su Empleabilidad en I+D+i” statal programProject
Gobierno de España. MAT2013–47395-C4–1-REditor's Version
http://dx.doi.org/10.1002/adma.201505020Subjects
Optical trapping; Luminescence nanothermometry; Plasmonic in vitro heating; FísicaRights
© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, WeinheimAbstract
Single particle spectroscopy in the form of three-dimensional optical manipulation of an upconverting nanoparticle is here used for non-invasive thermal sensing at the cellular level. In particular, a single infrared 980 nm laser beam is used as a three-dimensional optical tweezer and, simultaneously, as an optical excitation source for a single NaYF4:Er3+,Yb3+ upconverting particle. Real time analysis of the thermosensitive green emission of Er3+ ions obtained after Yb3+ excitation provides thermal sensing during optical manipulation. Thus, three-dimensional particle scanning allows for the measurement of thermal gradients in the surroundings of individual cancer cells subjected to a plasmonic-mediated photothermal therapy. It is found that such thermal gradients extends for distances larger than 10 microns, avoiding real single cell photothermal treatments under in vitro conditions. This work introduces to the scientific community a novel and simple approach for high resolution thermal sensing at the cellular level that could constitute a powerful tool for a better understanding of cell dynamics during thermal treatments
Files in this item
Google Scholar:Rodríguez-Sevilla, Paloma
-
Zhang, Yuhai
-
Haro González, Patricia
-
Sanz Rodríguez, Francisco
-
Jaque, Francisco
-
García Solé, José
-
Liu, Xiaogang
-
Jaque García, Daniel
This item appears in the following Collection(s)
Related items
Showing items related by title, author, creator and subject.