Improvement in glycemia after glucose or insulin overload in leptin-infused rats is associated with insulin-related activation of hepatic glucose metabolism
Entity
UAM. Departamento de Pediatría; Instituto de Investigación del Hospital de La Princesa (IP)Publisher
BioMed CentralDate
2016-03-01Citation
10.1186/s12986-016-0079-9
Nutrition & Metabolism 13.19 (2016): 1-6
ISSN
1743-7075DOI
10.1186/s12986-016-0079-9Funded by
This work was supported by the Spanish Ministry of Science and Innovation with the help of European FEDER funding (FIS PI13/02195), Ministerio de Ciencia e Innovación (BFU2011-27492 and BFU2014-51836-C2-2-R), the Network Center for Biomedical Research on Obesity and Nutrition (CIBEROBN) Instituto Carlos III and Fundación Endocrinología y Nutrición. S.C. is supported by CIBEROBNProject
Gobierno de España. BFU2011-27492; Gobierno de España. BFU2014-51836-C2-2-REditor's Version
http://dx.doi.org/10.1186/s12986-016-0079-9Subjects
Glycemia; Glycogen synthesis; Insulin signaling; Leptin; Liver; Tolerance test; MedicinaRights
© 2016 Burgos-Ramos et al.Abstract
Background: Insulin regulates glucose homeostasis through direct effects on the liver, among other organs, with leptin modulating insulin's hepatic actions. Since central leptin may modify insulin signaling in the liver, we hypothesized that leptin infusion activates hepatic glycogen synthesis following peripheral administration of a bolus of glucose or insulin, thus regulating glycemia. Findings: Oral glucose and intraperitoneal insulin tolerance tests were performed in control, intracerebroventricular leptin-treated and pair-fed rats during 14 days. An improvement in glycemia and an increase in hepatic free glucose and glycogen concentrations after glucose or insulin overload were observed in leptin-treated rats. In order to analyze whether the liver was involved in these changes, we studied activation of insulin signaling by Western blotting and multiplex bead immunoassay after leptin infusion. Our studies revealed an increase in phosphorylation of insulin receptor substrate-1 and Akt in leptin-treated rats. Examination of parameters related to glucose uptake and metabolism in the liver revealed an augment in glucose transporter 2 and a decrease in phosphoenolpyruvate carboxylase protein levels in this group. Conclusions: These results indicate that central leptin increases hepatic insulin signaling, associated with increased glycogen concentrations after glucose or insulin overload, leading to an improvement in glycemia.
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Google Scholar:Burgos-Ramos, Emma
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Canelles, Sandra
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Frago Fernández, Laura María
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Chowen, Julie Ann
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Arilla-Ferreiro, Eduardo
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Argente Oliver, Jesús
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Barrios, Vicente
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