Mañana, JUEVES, 24 DE ABRIL, el sistema se apagará debido a tareas habituales de mantenimiento a partir de las 9 de la mañana. Lamentamos las molestias.
Nitrogen fixation in a nonheterocystous cyanobacterial mat from a mountain river
Entity
UAM. Departamento de BiologíaPublisher
Nature Publishing GroupDate
2016-08-01Citation
10.1038/srep30920
Scientific Reports 6 (2016): 30920
ISSN
2045-2322DOI
10.1038/srep30920Funded by
This work was supported by grants from the Ministerio de Ciencia e Innovación and Ministerio de Economía y Competitividad, Spain (CGL2008-02397/BOS and CGL-2013-44870-R), and from the Comunidad Autónoma de Madrid (S2009/AMB-1511). We also thank Professor B A Whitton for helpful suggestions on the manuscript, and Dr Philip Mason for improvements to the English version of our manuscriptProject
Gobierno de España. CGL-2013-44870-R; Gobierno de España. CGL-2008-02397/BOS; Comunidad de Madrid. S2009/AMB-1511/MICROAMBIENTEEditor's Version
http://dx.doi.org/10.1038/srep30920Subjects
Schizothrix; Nitrogenase activity; 16S rRNA gene; Rivularia colonies; BotánicaRights
© The Author(s) 2016Abstract
In situ nitrogen fixation was investigated in a cyanobacterial mat growing on the bed of rocks of the Muga River, Spain. The filamentous non-heterocystous cyanobacterium Schizothrix dominated the mat, showing nitrogenase activity in the light at similar rates to those found in nearby heterocystous Rivularia colonies. N2 fixation in the light was significantly increased by an inhibitor of PSII and oxygen evolution, DCMU (3-[3,4-dichlorophenyl]-1,1-dimethylurea), and anaerobic conditions. However, no nitrogenase activity was found in the dark. Addition of fructose as a respiratory substrate induced nitrogenase activity in samples incubated under aerobic conditions in the dark but not in anaerobic conditions. Microelectrode oxygen profiles showed internal microaerobic microzones where nitrogen fixation might concentrate. Analyses of the 16S rRNA gene revealed only the presence of sequences belonging to filamentous non-heterocystous cyanobacteria. nifH gene diversity showed that the major phylotypes also belonged to this group. One of the three strains isolated from the Schizothrix mat was capable of fixing N2 and growing in the absence of combined N. This was consistent with the nifH gene analysis. These results suggest a relevant contribution of non-heterocystous cyanobacteria to nitrogen fixation in these mats
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Google Scholar:Berrendero, Esther
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Fernández Valiente, Eduardo
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Perona Urízar, Elvira Victoria
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Gómez, Claudia L.
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Loza, Virginia
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Muñoz-Martín, M. Ángeles
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Mateo Ortega, Pilar
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