Beyond chloride brines: Variable metabolomic responses in the anaerobic organism Yersinia intermedia MASE-LG-1 to NaCl and MgSO4 identical water activity
Author
Schwendner, Petra; Bohmeier, Maria; Rettberg, Petra; Beblo-Vranesevic, Kristina; Gaboyer, Frédéric; Moissl-Eichinger, Christine; Perras, Alexandra K.; Vannier, Pauline; Marteinsson, Viggo T.; Garcia-Descalzo, Laura; Gómez, Felipe; Malki, Moustafa; Amils Pibernat, Ricardo; Westall, Frances; Riedo, Andreas; Monaghan, Euan P.; Ehrenfreund, Pascale; Cabezas, Patricia; Walter, Nicolas; Cockell, CharlesEntity
UAM. Departamento de Biología Molecular; Centro de Biología Molecular Severo Ochoa (CBM)Publisher
Frontiers MediaDate
2018-02-27Citation
10.3389/fmicb.2018.00335
Frontiers in Microbiology 9.February (2018): Article 335
ISSN
1664-302XDOI
10.3389/fmicb.2018.00335Funded by
MASE is supported by European Community’s Seventh Framework Program (FP7/2007-013) under Grant Agreement n◦ 607297Project
info:eu-repo/grantAgreement/EC/FP7/607297Editor's Version
https://doi.org/10.3389/fmicb.2018.00335Subjects
Compatible solutes; Magnesium sulfate; Metabolome; Sodium chloride; Stress response; Biología y Biomedicina / BiologíaRights
© 2018 Schwendner, Bohmeier, Rettberg, Beblo-Vranesevic, Gaboyer, Moissl-Eichinger, Perras, Vannier, Marteinsson, Garcia-Descalzo, Gómez, Malki, Amils, Westall, Riedo, Monaghan, Ehrenfreund, Cabezas, Walter and Cockell.Abstract
Growth in sodium chloride (NaCl) is known to induce stress in non-halophilic microorganisms leading to effects on the microbial metabolism and cell structure. Microorganisms have evolved a number of adaptations, both structural and metabolic, to counteract osmotic stress. These strategies are well-understood for organisms in NaCl-rich brines such as the accumulation of certain organic solutes (known as either compatible solutes or osmolytes). Less well studied are responses to ionic environments such as sulfate-rich brines which are prevalent on Earth but can also be found on Mars. In this paper, we investigated the global metabolic response of the anaerobic bacterium Yersinia intermedia MASE-LG-1 to osmotic salt stress induced by either magnesium sulfate (MgSO 4 ) or NaCl at the same water activity (0.975). Using a non-targeted mass spectrometry approach, the intensity of hundreds of metabolites was measured. The compatible solutes L-asparagine and sucrose were found to be increased in both MgSO 4 and NaCl compared to the control sample, suggesting a similar osmotic response to different ionic environments. We were able to demonstrate that Yersinia intermedia MASE-LG-1 accumulated a range of other compatible solutes. However, we also found the global metabolic responses, especially with regard to amino acid metabolism and carbohydrate metabolism, to be salt-specific, thus, suggesting ion-specific regulation of specific metabolic pathways
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Google Scholar:Schwendner, Petra
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Bohmeier, Maria
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Rettberg, Petra
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Beblo-Vranesevic, Kristina
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Gaboyer, Frédéric
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Moissl-Eichinger, Christine
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Perras, Alexandra K.
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Vannier, Pauline
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Marteinsson, Viggo T.
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Garcia-Descalzo, Laura
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Gómez, Felipe
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Malki, Moustafa
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Amils Pibernat, Ricardo
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Westall, Frances
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Riedo, Andreas
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Monaghan, Euan P.
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Ehrenfreund, Pascale
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Cabezas, Patricia
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Walter, Nicolas
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Cockell, Charles
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