Priming of dendritic cells by DNA-containing extracellular vesicles from activated T cells through antigen-driven contacts
Metadatos
Title:
Priming of dendritic cells by DNA-containing extracellular vesicles from activated T cells through antigen-driven contacts
Author:
Torralba, Daniel; Baixauli, Francesc; Villarroya Beltri, Carolina; Fernández-Delgado, Irene; Latorre-Pellicer, Ana; Acín-Pérez, Rebeca; Martín-Cófreces, Noa; Jaso-Tamame, Ángel Luis; Iborra, Salvador; Jorge, Inmaculada; González-Aseguinolaza, Gloria; Garaude, Johan; Vicente-Manzanares, Miguel; Enríquez, José Antonio; Mittelbrunn, María; Sánchez-Madrid, Francisco
Entity:
UAM. Departamento de Medicina; Instituto de Investigación Sanitaria Hospital Universitario de La Princesa (IIS-IP)
UAM Author:
Mittelbrum Herrero, María
; Villarroya Beltrí, Carolina
; Sánchez Madrid, Francisco
Publisher:
Nature Research (part of Springer Nature)
Date:
2018-07-09
Citation:
10.1038/s41467-018-05077-9
Nature Communications 9.1 (2018): 2658
ISSN:
2041-1723
DOI:
10.1038/s41467-018-05077-9
Funded by:
This study was supported by grants SAF2017/82886-R from the
Spanish Ministry of Economy and Competitiveness, CAM S2017/BMD-3671 from the
Comunidad de Madrid, CIBER Cardiovascular (Fondo de Investigación Sanitaria del
Instituto de Salud Carlos III and co-funding by Fondo Europeo de Desarrollo Regional
FEDER), ERC-2011-AdG 294340-GENTRIS and COST-Action BM1202 to F.S.-M.; grant
SAF2015-65633-R from the Spanish Ministry of Economy and Competitiveness to J.A.E.
M.M. is supported by MS14/00219 from Instituto de Salud Carlos III. Centro Nacional de
Investigaciones Cardiovasculares (CNIC) is supported by the Spanish Ministry of
Economy and Competitiveness (MINECO) and the Pro-CNIC Foundation, and is a
Severo Ochoa Center of Excellence (MINECO award SEV-2015-0505).
Project:
Gobierno de España. SAF2017/82886-R; Comunidad de Madrid. S2017/BMD-3671/INFLAMUNE; Gobierno de España. ERC-2011-AdG 294340-GENTRIS; COST-Action BM1202
Editor's Version:
https://doi.org/10.1038/s41467-018-05077-9
Subjects:
T cell; Dendritic cells (DC; Extracellular vesicles; DNA; Contacts; Medicina
Rights:
© 2018 The Author(s)
Abstract:
Interaction of T cell with antigen-bearing dendritic cells (DC) results in T cell activation, but whether this interaction has physiological consequences on DC function is largely unexplored. Here we show that when antigen-bearing DCs contact T cells, DCs initiate anti-pathogenic programs. Signals of this interaction are transmitted from the T cell to the DC, through extracellular vesicles (EV) that contain genomic and mitochondrial DNA, to induce antiviral responses via the cGAS/STING cytosolic DNA-sensing pathway and expression of IRF3-dependent interferon regulated genes. Moreover, EV-treated DCs are more resistant to subsequent viral infections. In summary, our results show that T cells prime DCs through the transfer of exosomal DNA, supporting a specific role for antigen-dependent contacts in conferring protection to DCs against pathogen infection. The reciprocal communication between innate and adaptive immune cells thus allow efficacious responses to unknown threats
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