Mañana, JUEVES, 24 DE ABRIL, el sistema se apagará debido a tareas habituales de mantenimiento a partir de las 9 de la mañana. Lamentamos las molestias.
The transcriptome of the Bermuda fireworm Odontosyllis enopla (Annelida: Syllidae): A unique luciferase gene family and putative epitoky-related genes
Entity
UAM. Departamento de BiologíaPublisher
Public Library of ScienceDate
2018-08-08Citation
10.1371/journal.pone.0200944
Plos One 13.8 (2018):e0200944
ISSN
1932-6203DOI
10.1371/journal.pone.0200944Funded by
The Gerstner Family Foundation provided partial support to MRB. MTA received support as the PI of the project “Macroevolutionary transitions in Syllidae” CGL2015-63593-P from MINECO/FEDER, UE funds. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. There was no additional external funding received for this studyProject
Gobierno de España. CGL2015-63593-PEditor's Version
https://doi.org/10.1371/journal.pone.0200944Subjects
Odontosyllis enopla; Bioluminescence; Luciferase; Biología y Biomedicina / BiologíaRights
© 2018 Brugler et alAbstract
The Bermuda fireworm Odontosyllis enopla exhibits an extremely tight circalunar circadian
behavior that results in an impressive bioluminescent mating swarm, thought to be due to a
conventional luciferase-mediated oxidation of a light-emitting luciferin. In addition, the four
eyes become hypertrophied and heavily pigmented, and the nephridial system is modified
to store and release gametes and associated secretions. In an effort to elucidate transcripts
related to bioluminescence, circadian or circalunar periodicity, as well as epitoky-related
changes of the eyes and nephridial system, we examined the transcriptomic profile of three
female O. enopla during a bioluminescent swarm in Ferry Reach, Bermuda. Using the wellcharacterized luciferase gene of the Japanese syllid Odontosyllis undecimdonta as a reference, a complete best-matching luciferase open reading frame (329 amino acids in length)
was found in all three individuals analyzed in addition to numerous other paralogous
sequences in this new gene family. No photoproteins were detected. We also recovered a
predicted homolog of 4-coumarate-CoA ligase (268 amino acids in length) that best
matched luciferase of the firefly Luciola with the best predicted template being the crystal
structure of luciferase for Photinus pyralis, the common eastern firefly. A wide variety of
genes associated with periodicity were recovered including predicted homologs of clock,
bmal1, period, and timeless. Several genes corresponding to putative epitoky-related
changes of the eyes were recovered including predicted homologs of a phototransduction
gene, a retinol dehydrogenase and carotenoid isomerooxygenase as well as a visual perception related retinal rod rhodopsin-sensitive cGMP 3’,5’-cyclic phosphodiesterase. Genes
correlating to putative epitoky-related changes of the nephridia included predicted homologs
of nephrocystin-3 and an egg-release sex peptide receptor
Files in this item
Google Scholar:Brugler, Mercer R.
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Aguado Molina, María Teresa
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Tessler, Michael
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Sidall, Mark E.
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