Measurement of local resolution in electron tomography
Entity
UAM. Departamento de Ingeniería InformáticaPublisher
ElsevierDate
2020-01-01Citation
10.1016/j.yjsbx.2019.100016
Journal of Structural Biology: X 4(2020): 100016
ISSN
2590-1524DOI
10.1016/j.yjsbx.2019.100016Funded by
The author would like to express their gratitude to Dr. J.J. Fernandez for his interest, following and advice on this work. The authors would like to acknowledge economical support from The Spanish Ministry of Economy and Competitiveness through Grants BIO2016- 76400-R(AEI/FEDER, UE), the “Comunidad Autónoma de Madrid” through Grant: S2017/BMD-3817, and the European Union (EU) and Horizon 2020 through grant EOSCpilot (INFRADEV-04-2016, Proposal: 739563), INSTRUCT - ULTRA (INFRADEV-03-2016-2017, Proposal: 731005), EOSC-LIFE (INFRAEOSC-04-2018, Proposal: 824087) and HighResCells (ERC-2018-SyG, Proposal: 810057). The authors acknowledge the support and the use of resources of Instruct-ERICProject
Gobierno de España. BIO2016- 76400-R; Comunidad de Madrid. S2017/BMD-3817/TomoXliver-CM; info:eu-repo/grantAgreement/EC/H2020/739563/EU/EOSCpilot; info:eu-repo/grantAgreement/EC/H2020/731005/EU/INSTRUCT-ULTRA; info:eu-repo/grantAgreement/EC/H2020/824087/EU/EOSC-LIFE; info:eu-repo/grantAgreement/EC/H2020/810057/ERC/HighResCellsEditor's Version
http://doi.org/10.1016/j.yjsbx.2019.100016Subjects
Cryoem; Electron tomography; Image processing; Local resolution; InformáticaRights
© ElsevierAbstract
Resolution (global and local) is one of the most reported metrics of quality measurement in Single Particle Analysis (SPA). However, in electron tomography, the situation is different and its computation is not straightforward. Typically, resolution estimation is global and, therefore, reduces the assessment of a whole tomogram to a single number. However, it is known that tomogram quality is spatially variant. Still, up to our knowledge, a method to estimate local quality metrics in tomography is lacking. This work introduces MonoTomo, a method developed to estimate locally in a tomogram the highest reliable frequency component, expressed as a form of local resolution. The fundamentals lie in a local analysis of the density map via monogenic signals, which, in analogy to MonoRes, allows for local estimations. Results with experimental data show that the local resolution range that MonoTomo casts agrees with reported resolution values for experimental data sets, with the advantage of providing a local estimation. A range of applications of MonoTomo are suggested for further exploration
Files in this item
Google Scholar:Vilas, J. L.
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Oton, J.
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Messaoudi, C.
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Melero, R.
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Conesa, P.
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Ramirez-Aportela, E.
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Mota, J.
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Martinez, M.
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Jimenez, A.
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Marabini Ruiz, Roberto
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Carazo, J. M.
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Vargas, J.
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Sorzano, C. O.S.
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