Circulating extracellular vesicle proteins and microRNA profiles in subcortical and cortical-subcortical ischaemic stroke
Author
Otero-Ortega, Laura; Alonso-López, Elisa; Pérez-Mato, María; Laso-García, Fernando; Gómez-De Frutos, Mari Carmen; Diekhorst, Luke; García-Bermejo, María Laura; Conde-Moreno, Elisa; Fuentes Gimeno, Blanca Eulalia; de Leciñana, María Alonso; Bravo, Susana B.; Díez Tejedor, Exuperio; Gutiérrez-Fernández, MaríaEntity
UAM. Departamento de Enfermería; UAM. Departamento de MedicinaPublisher
MDPIDate
2021-07-07Citation
10.3390/biomedicines9070786
Biomedicines 9.7 (2021): 786
ISSN
2227-9059DOI
10.3390/biomedicines9070786Funded by
This work was sponsored by a grant from Miguel Servet (CP15/00069; CPII20/00002 to María Gutiérrez-Fernández), Miguel Servet (CP20/00024 to Laura Otero-Ortega), a predoctoral fellowship (FI17/00188 to Mari Carmen Gómez-de Frutos; FI18/00026 to Fernando Laso-García), a Sara Borrell postdoctoral fellowship (CD19/00033 to María Pérez-Mato), a Río Hortega (CM20/00047 to Elisa Alonso-López) and the INVICTUS PLUS network grant (RD16/0019/0005) from the Carlos III Health Institute Health Care Research Fund and was co-funded by the European Regional Development Fund (ERDF).Project
Gobierno de España. RD16/0019/0005Subjects
Exosomes; Extracellular vesicles; Ischaemic stroke; MiRNA; Proteomic analysis; MedicinaRights
© 2021 by the authorsAbstract
In order to investigate the role of circulating extracellular vesicles (EVs), proteins, and microRNAs as damage and repair markers in ischaemic stroke depending on its topography, subcor-tical (SC), and cortical-subcortical (CSC) involvement, we quantified the total amount of EVs using an enzyme-linked immunosorbent assay technique and analysed their global protein content using proteomics. We also employed a polymerase chain reaction to evaluate the circulating microRNA profile. The study included 81 patients with ischaemic stroke (26 SC and 55 CSC) and 22 healthy controls (HCs). No differences were found in circulating EV levels between the SC, CSC, and HC groups. We detected the specific expression of C1QA and Casp14 in the EVs of patients with CSC ischaemic stroke and the specific expression of ANXA2 in the EVs of patients with SC involvement. Patients with CSC ischaemic stroke showed a lower expression of miR-15a, miR-424, miR-100, and miR-339 compared with those with SC ischaemic stroke, and the levels of miR-339, miR-100, miR-199a, miR-369a, miR-424, and miR-15a were lower than those of the HCs. Circulating EV proteins and microRNAs from patients with CSC ischaemic stroke could be considered markers of neurite outgrowth, neurogenesis, inflammation process, and atherosclerosis. On the other hand, EV proteins and microRNAs from patients with SC ischaemic stroke might be markers of an anti-inflammatory process and blood–brain barrier disruption reduction.
Files in this item
Google Scholar:Otero-Ortega, Laura
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Alonso-López, Elisa
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Pérez-Mato, María
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Laso-García, Fernando
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Gómez-De Frutos, Mari Carmen
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Diekhorst, Luke
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García-Bermejo, María Laura
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Conde-Moreno, Elisa
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Fuentes Gimeno, Blanca Eulalia
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de Leciñana, María Alonso
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Bravo, Susana B.
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Díez Tejedor, Exuperio
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Gutiérrez-Fernández, María
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