Carbon nanodots modified-electrode for peroxide-free cholesterol biosensing and biofuel cell design
Entity
UAM. Departamento de Química Analítica y Análisis InstrumentalPublisher
ElsevierDate
2022-10-25Citation
10.1016/j.snb.2022.132895
Sensors and Actuators: B. Chemical 375 (2023): 132895
ISSN
0925-4005 (print)DOI
10.1016/j.snb.2022.132895Funded by
A.L.DL. and M.P. thank MCIU/AEI/FEDER, EU for funding project RTI2018–095090-B-I00. M.B. acknowledges funding from the European Union’s Horizon 2020 Research and Innovation Program under the Marie Skłodowska-Curie grant agreement No. 713366. This work was also supported by Talent Attraction Project from CAM (SI3/PJI/ 2021–00341 and 2021–5A/BIO-20943), Spanish Ministerio de Ciencia e Innovacion (PID2020–116728RB-I00) and TRANSNANOAVANSENSCAM Program (S2018/NMT-4349)Project
Gobierno de España. PID2020–116728RB-I00Editor's Version
https://doi.org/10.1016/j.snb.2022.132895Subjects
Biofuel cell; Biosensor; Carbon nanodots; Cholesterol; Dehydrogenase; QuímicaRights
© 2022 The AuthorsEsta obra está bajo una licencia de Creative Commons Reconocimiento-NoComercial-SinObraDerivada 4.0 Internacional.
Abstract
The determination of cholesterol is greatly important because high concentrations of this biomarker are associated to heart disease. Moreover, cholesterol can be used as a fuel in enzymatic fuel cells operating under physiological conditions. Here, we present a cholesterol biosensor and a peroxide-free biofuel cell based on the electrocatalytic oxidation of the NADH generated during the enzymatic reaction of cholesterol dehydrogenase (ChDH) as an alternative to the H2O2 biosensing strategies used with cholesterol oxidase-bioelectrodes. Azure A functionalized-carbon nanodots were used as NADH oxidation electrocatalysts and for ChDH covalent immobilization. The biosensor responded linearly to cholesterol concentrations up to 1.7 mM with good sensitivity (4.50 mA cm−2 M−1) and at a low potential. The ChDH bioelectrode was combined with an O2-reducing bilirubin oxidase cathode to produce electrical energy using cholesterol as fuel and O2 as oxidant. Furthermore, the resulting enzymatic fuel cell was tested in human serum naturally containing free cholesterol
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Google Scholar:Barrio Redondo, Melisa del
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Martínez Periñán, Emiliano
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Gutiérrez Sánchez, María Cristina
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Mateo Martí, Eva
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Pita, Marcos
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De Lacey, Antonio L.
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Pariente Alonso, Félix
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Lorenzo Abad, Encarnación
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