Nitric oxide and superoxide anion balance in rats exposed to chronic and long term intermittent hypoxia
Entidad
UAM. Departamento de Fisiología; UAM. Departamento de Medicina Preventiva y Salud Pública y MicrobiologíaEditor
Hindawi Publishing CorporationFecha de edición
2014-01-01Cita
10.1155/2014/610474
BioMed Research International 2014 (2014): 610474
ISSN
2314-6133 (print); 2255-1042 (online)DOI
10.1155/2014/610474Financiado por
This work was funded by GORE-TARAPACA (BIP 30125349- 0) and ALTMEDFIS (CYTED 213RT0478) GrantsVersión del editor
http://dx.doi.org/10.1155/2014/610474Materias
Nitric oxide; Hypoxia; MedicinaDerechos
Copyright © 2014 Patricia Siques et al.Resumen
Work at high altitude in shifts exposes humans to a new form of chronic intermittent hypoxia, with still unknown health
consequences. We have established a rat model resembling this situation, which develops a milder form of right ventricular
hypertrophy and pulmonary artery remodelling compared to continuous chronic exposure. We aimed to compare the alterations
in pulmonary artery nitric oxide (NO) availability induced by these forms of hypoxia and the mechanisms implicated. Rats were
exposed for 46 days to normoxia or hypobaric hypoxia, either continuous (CH) or intermittent (2 day shifts, CIH2x2), and
assessed: NO and superoxide anion availability (fluorescent indicators and confocal microscopy); expression of phosphorylated
endothelial NO synthase (eNOS), NADPH-oxidase (p22phox), and 3-nitrotyrosine (western blotting); and NADPH-oxidase
location (immunohistochemistry). Compared to normoxia, (1) NO availability was reduced and superoxide anion was increased in
both hypoxic groups, with a larger effect in CH, (2) eNOS expression was only reduced in CH, (3) NADPH-oxidase was similarly
increased in both hypoxic groups, and (4) 3-nitrotyrosine was increased to a larger extent inCH. In conclusion, intermittent hypoxia
reduces NO availability through superoxide anion destruction, without reducing its synthesis, while continuous hypoxia affects
both, producing larger nitrosative damage which could be related to the more severe cardiovascular alterations
Lista de ficheros
Google Scholar:Siqués, Patricia
-
López de Pablo León, Ángel Luis
-
Brito, Julio P.
-
Arribas Rodríguez, Silvia Magdalena
-
Flores, Karen
-
Arriaza, Karem
-
Naveas, Nelson
-
González, Maria Carmen
-
Hoorntje, Alexander
-
León-Velarde, Fabiola
-
López, Mercedes Rosario
Lista de colecciones del ítem
Registros relacionados
Mostrando ítems relacionados por título, autor, creador y materia.