Nanostructured porous silicon micropatterns as a tool for substrate-conditioned cell research
Entity
UAM. Departamento de Física AplicadaPublisher
Springer New YorkDate
2012-07-16Citation
10.1186/1556-276X-7-396
Nanoscale Research Letters 7(2011): 1-7
ISSN
1931-7573 (print); 1556-276X (online)DOI
10.1186/1556-276X-7-396Funded by
The authors gratefully acknowledge the financial support from MICINN under research project MAT2008-06858-C02-01/NAN and Comunidad de Madrid (Spain) under Project Microseres. Technical support from L García Pelayo is greatly appreciatedProject
Comunidad de Madrid. S2009/TIC-1476/MICROSERESEditor's Version
http://dx.doi.org/10.1186/1556-276X-7-396Subjects
Cell adhesion; Cell guide; Human mesenchymal stem cells; Ion beam; Micropattern; Nanostructured porous silicon; Photoluminescence; FísicaRights
© 2012 Punzón-Quijorna et al.Abstract
The localized irradiation of Si allows a precise patterning at the microscale of nanostructured materials such as porous silicon (PS). PS patterns with precisely defined geometries can be fabricated using ion stopping masks. The nanoscale textured micropatterns were used to explore their influence as microenvironments for human mesenchymal stem cells (hMSCs). In fact, the change of photoluminescence emission from PS upon aging in physiological solution suggests the intense formation of silanol surface groups, which may play a relevant role in ulterior cell adhesion. The experimental results show that hMSCs are sensitive to the surface micropatterns. In this regard, preliminary β-catenin labeling studies reveal the formation of cell to cell interaction structures, while microtubule orientation is strongly influenced by the selective adhesion conditions. Relevantly, Ki-67 assays support a proliferative state of hMSCs on such nanostructured micropatterns comparable to that of standard cell culture platforms, which reinforce the candidature of porous silicon micropatterns to become a conditioning structure for in vitro culture of hMSCs
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Google Scholar:Punzón Quijorna, Esther
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Sánchez-Vaquero, Vanessa
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Muñoz Noval, Álvaro
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Pérez-Roldán, Maria Jesús
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Martín Palma, Raúl José
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Rossi, François
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Climent Font, Aurelio
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Manso Silván, Miguel
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García-Ruíz, Josefa Predestinación
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Torres Costa, Vicente
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