Stereological analysis of neuron, glial and endothelial cell numbers in the human amygdaloid complex
Entidad
UAM. Departamento de Anatomía, Histología y NeurocienciaEditor
Public Library of ScienceFecha de edición
2012-06-13Cita
10.1371/journal.pone.0038692
Plos One 7.6 (2012): e38692
ISSN
1932-6203 (online)DOI
10.1371/journal.pone.0038692Financiado por
The project was supported by Grants from the Spanish Fondo de Investigación Sanitaria del Instituto de Salud Carlos III (PI070199)(http://www.isciii.es/) and the Fundación Eugenio Rodríguez Pascual (http://www.fundacioneugeniorodriguezpascual.es/), and a predoctoral fellowship from the Universidad Autónoma de Madrid (FPI2009) (www.uam.es) to María García-AmadoMaterias
Acetylcholinesterase; Amygdala; Endothelium; Immunohistochemistry; Neuroglia; Neurons; MedicinaDerechos
© 2012 García -Amado, PrensaResumen
Cell number alterations in the amygdaloid complex (AC) might coincide with neurological and psychiatric pathologies with anxiety imbalances as well as with changes in brain functionality during aging. This stereological study focused on estimating, in samples from 7 control individuals aged 20 to 75 years old, the number and density of neurons, glia and endothelial cells in the entire AC and in its 5 nuclear groups (including the basolateral (BL), corticomedial and central groups), 5 nuclei and 13 nuclear subdivisions. The volume and total cell number in these territories were determined on Nissl-stained sections with the Cavalieri principle and the optical fractionator. The AC mean volume was 956 mm3 and mean cell numbers (x106) were: 15.3 neurons, 60 glial cells and 16.8 endothelial cells. The numbers of endothelial cells and neurons were similar in each AC region and were one fourth the number of glial cells. Analysis of the influence of the individuals' age at death on volume, cell number and density in each of these 24 AC regions suggested that aging does not affect regional size or the amount of glial cells, but that neuron and endothelial cell numbers respectively tended to decrease and increase in territories such as AC or BL. These accurate stereological measures of volume and total cell numbers and densities in the AC of control individuals could serve as appropriate reference values to evaluate subtle alterations in this structure in pathological conditions
Lista de ficheros
Google Scholar:García-Amado, María
-
Prensa Sepúlveda, Lucía
Lista de colecciones del ítem
Registros relacionados
Mostrando ítems relacionados por título, autor, creador y materia.