Augmentation of catecholamine release elicited by an Eugenia punicifolia extract in chromaffin cells
Title (trans.)
Brazilian Journal of PharmacognosyEntity
UAM. Departamento de FarmacologíaPublisher
Sociedade Brasileira de FarmacognosiaDate
2011-01-01Citation
10.1590/S0102-695X2011005000191
Revista Brasileira de Farmacognosia 22.1 (2012): 1-12
ISSN
0102-695X (print); 1981-528X (online)DOI
10.1590/S0102-695X2011005000191Funded by
This work was partially supported by grants from the Ministerio de Ciencia e Innovación (SAF2010- 21795 to AGG, SAF2010-18837 to LG, and SAB2006-0123 to WCS), Comunidad Autónoma de Madrid (S-SAL-0275-2006), and CEAL-SANTANDER (to LG). We thank the Fundación Teófilo Hernando (Spain) and FAPERJ (Brasil) for funding and continued supportProject
Gobierno de España. SAF2010-21795; Gobierno de España. SAF2010-18837; Gobierno de España. SAB2006-0123; Comunidad de Madrid. S2006/SAL-0275Editor's Version
http://dx.doi.org/10.1590/S0102- 695X2011005000191Subjects
Calcium channels; Catecholamine release; Chromaffin cells; Eugenia punicifolia; Nicotinic receptors; FarmaciaAbstract
Plant extracts of Eugenia punicifolia (Kunth) DC., Myrtaceae, are
used in Amazon region of Brazil to treat diarrhea and stomach disturbances,
and as hypoglycemic medicine. We have recently shown that an aqueous extract
of E. punicifolia augmented cholinergic neurotransmission in a rat phrenic
nerve-diaphragm preparation. In this study, we investigated the effects of an E.
punicifolia dichloromethane extract (EPEX) in a neuronal model of cholinergic
neurotransmission, the bovine adrenal chromaffin cell. EPEX augmented the release
of catecholamine triggered by acetylcholine (ACh) pulses but did not enhance AChevoked
inward currents, which were inhibited by 30%. Since EPEX did not cause
a blockade of acetylcholinesterase or butyrylcholinesterase, it seems that EPEX is
not directly activating the cholinergic system. EPEX also augmented K+-elicited
secretion without enhancing the whole-cell inward calcium current. This novel and
potent effect of EPEX in enhancing exocytosis might help to identify the active
component responsible for augmenting exocytosis. When elucidated, the molecular
structure of this active principle could serve as a template to synthesise novel
compounds to regulate the exocytotic release of neurotransmitters
Files in this item
Google Scholar:Pascual, Ricardo de
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Colmena, Inés
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Ríos, Cristóbal de los
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Rosa, Jualiana M.
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Correa-Leite, Paulo E.
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Lim-Araújo, Kátia
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Ferreira, Vitor F.
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Rocha, David R.
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Gonzaga, Daniel T.
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García, Antonio G.
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Santos, Wilson C.
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Gandía Juan, Luis
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