Adventitial alterations are the main features in pulmonary artery remodeling due to long-term chronic intermittent hypobaric hypoxia in rats
Entity
UAM. Departamento de Fisiología; UAM. Departamento de Medicina Preventiva y Salud Pública y MicrobiologíaPublisher
Hindawi Publishing CorporationDate
2015-01-01Citation
10.1155/2015/169841
BioMed Research International 2015 (2015): 169841
ISSN
2314-6133DOI
10.1155/2015/169841Funded by
This work was funded by GORE-FNDR-BIP: 30125349-0, AECID Nº A/030023/10, and CYTED-ALTMEDFIS grantsEditor's Version
http://dx.doi.org/10.1155/2015/169841Subjects
Hipoxia; Pulmonary artery; MedicinaRights
© 2015 Julio Brito et al.Abstract
Long-termchronic intermittent exposure to altitude hypoxia is a labor phenomenon requiring further research. Using a rat model, we examined whether this type of exposure differed from chronic exposure in terms of pulmonary artery remodeling and other features. Rats were subjected to chronic hypoxia (CH, 𝑛 = 9) and long-term intermittent hypoxia (CIH2x2; 2 days of hypoxia/2 days of normoxia, 𝑛 = 10) in a chamber (428 Torr, 4,600m of altitude) for 46 days and compared to rats under normoxia (NX, 𝑛 = 10). Body weight, hematocrit, and right ventricle ratio were measured. Pulmonary artery remodeling was assessed using confocal microscopy of tissues stained with a nuclear dye (DAPI) and CD11b antibody. Both hypoxic conditions exhibited increased hematocrit and hypertrophy of the right ventricle, tunica adventitia, and tunica media, with no changes in lumen size. The medial hypertrophy area (larger in CH) depicted a significant increase in smooth muscle cell number. Additionally, CIH2x2 increased the adventitial hypertrophy area, with an increased cellularity and a larger prevalence of clustered inflammatory cells. In conclusion, CIH2x2 elicitsmilder effects on pulmonary artery medial layermuscularization and subsequent right ventricular hypertrophy than CH. However, CIH2x2 induces greater and characteristic alterations of the adventitial layer
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Google Scholar:Brito, Julio P.
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Siques, Patricia
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Arribas Rodríguez, Silvia Magdalena
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López de Pablo León, Ángel Luis
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González Enguita, María del Carmen
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Naveas, Nelson
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Arriaza, Karem
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Flores, Karen
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León-Velarde, Fabiola
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Pulido, Ruth
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Ordenes, Stefany
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López, M. Rosario
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