Novel synthetic sulfoglycolipid IG20 facilitates exocytosis in chromaffin cells through the regulation of sodium channels
Entity
UAM. Departamento de FarmacologíaPublisher
John Wiley & Sons, IncDate
2015-12-01Citation
10.1111/jnc.13357
Journal of Neurochemistry 135.5 (2015): 880-896
ISSN
0022-3042; 0022-3042DOI
10.1111/jnc.13357Funded by
This work was supported by grants from Ministerio de Economía y Competitividad, Spain (MINECO, SAF-2013-44108-P to AGG and LG; and FIS-PI11/01436 to AFM), Fundación Eugenio Rodríguez Pascual (to LG). We thank the continued support of Fundación Teófilo Hernando, Madrid, SpainProject
Gobierno de España. SAF-2013-44108-P; Gobierno de España. FIS-PI11/01436Editor's Version
http://dx.doi.org/10.1111/jnc.13357Subjects
Compound IG20; Exocytosis; Sodium channel; Calcium channel; Sulfoglycolipid; Chromaffin cell; MedicinaNote
This is the peer reviewed version of the following article: Novel synthetic sulfoglycolipid IG20 facilitates exocytosis in chromaffin cells through the regulation of sodium channels: Journal of Neurochemistre, 135.5 (2015), which has been published in final form at http://dx.doi.org/10.1111/jnc.13357Rights
© 2015 International Society for NeurochemistryAbstract
In searching for druggable synthetic lipids as potential modulators of synaptic transmission and
plasticity, we synthesized sulfoglycolipid IG20 that stimulates neuritic outgrowth. Here we have
explored its effects on ion channels and exocytosis in bovine chromaffin cells (BCCs). IG20
augmented the rate of basal catecholamine release. Such effect did not depend on Ca2+ mobilization
from intracellular stores; rather, IG20-elicited secretion entirely dependent on Ca2+ entry through Lsubtype
voltage-activated Ca2+ channels. Those channels were recruited by cell depolarization
mediated by IG20 likely through its ability to enhance the recruitment of Na+ channels at more
hyperpolarizing potentials. Confocal imaging with fluorescent derivative IG20-NBD revealed its rapid
incorporation and confinement into the plasmalemma, supporting the idea that IG20 effects are exerted
through a plasmalemmal-delimited mechanism. Thus, synthetic IG20 seems to mimic several
physiological effects of endogenous lipids such as regulation of ion channels, Ca2+ signaling, and
exocytosis. Therefore, sulfoglycolipid IG20 may become a pharmacological tool to investigate the role
of the lipid environment on neuronal excitability, ion channels, neurotransmitter release, synaptic
efficacy, and neuronal plasticity. It may also inspire the synthesis of druggable sulfoglycolipids aimed
at increasing synaptic plasticity and efficacy in neurodegenerative diseases and traumatic brain –
spinal cord injury
Files in this item
Google Scholar:Crespo-Castrillo, Andrea
-
Punzón, Eva
-
Pascual, Ricardo de
-
Maroto, Marcos
-
Padín, Juan Fernando
-
García-Álvarez, Isabel
-
Nanclares, Carmen
-
Ruiz-Pascual, Lucía
-
Gandía Juan, Luis
-
Fernández-Mayoralas, Alfonso
-
García, Antonio G.
This item appears in the following Collection(s)
Related items
Showing items related by title, author, creator and subject.