Transposase interaction with the β sliding clamp: Effects on insertion sequence proliferation and transposition rate
Entidad
UAM. Departamento de Biología MolecularEditor
Nature Publishing GroupFecha de edición
2015-08-26Cita
10.1038/srep13329
Scientific Reports 5 (2015): 13329
ISSN
2045-2322DOI
10.1038/srep13329Financiado por
This work was funded by the Subdirección General de Proyectos de Investigación of the Spanish Ministry for Economy and Competitiveness Grants No. CGL2010-17384 and AYA2011-24803, and by the European Research Council (ERC) Advanced Grant No. 250350 (IPBSL). HDM has a FPI fellowship from the Spanish GovernmentProyecto
Gobierno de España. CGL2010-17384; Gobierno de España. AYA2011-24803; info:eu-repo/grantAgreement/EC/FP7/250350Versión del editor
http://dx.doi.org/10.1038/srep13329Materias
Insertion sequences; Transposase; Bacterial genomes; Potential of ISs; Chromosomes.; Biología y Biomedicina / BiologíaEsta obra está bajo una licencia de Creative Commons Reconocimiento-NoComercial-SinObraDerivada 4.0 Internacional.
Resumen
Insertion sequences (ISs) are ubiquitous and abundant mobile genetic elements in prokaryotic genomes. ISs often encode only one protein, the transposase, which catalyzes their transposition. Recent studies have shown that transposases of many different IS families interact with the β sliding clamp, a DNA replication factor of the host. However, it was unclear to what extent this interaction limits or favors the ability of ISs to colonize a chromosome from a phylogenetically-distant organism, or if the strength of this interaction affects the transposition rate. Here we describe the proliferation of a member of the IS1634 family in Acidiphilium over ~600 generations of cultured growth. We
demonstrate that the purified transposase binds to the β sliding clamp of Acidiphilium, Leptospirillum and E. coli. Further, we also demonstrate that the Acidiphilium IS1634 transposase binds to
the archaeal sliding clamp (PCNA) from Methanosarcina, and that the transposase encoded by Methanosarcina IS1634 binds to Acidiphilium β. Finally, we demonstrate that increasing the strength of the interaction between β and transposase results in a higher transposition rate in vivo. Our results suggest that the interaction could determine the potential of ISs to be mobilized in bacterial
populations and also their ability to proliferate within chromosomes
Lista de ficheros
Google Scholar:Díaz-Maldonado, Héctor
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Gómez, Manuel J.
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Moreno-Paz, Mercedes
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San Martín-Úriz, Patxi
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Amils Pibernat, Ricardo
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Parro, Víctor
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López de Saro, Francisco J.
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