Mañana, JUEVES, 24 DE ABRIL, el sistema se apagará debido a tareas habituales de mantenimiento a partir de las 9 de la mañana. Lamentamos las molestias.
Wavelet Imaging on Multiple Scales (WIMS) reveals focal adhesion distributions, dynamics and coupling between actomyosin bundle stability
Entidad
UAM. Departamento de Medicina; Instituto de Investigación del Hospital de La Princesa (IP)Editor
Public Library of ScienceFecha de edición
2017-10-19Cita
10.1371/journal.pone.0186058
PLoS ONE 12.10 (2017): e0186058
ISSN
1932-6203DOI
10.1371/journal.pone.0186058Financiado por
This work was supported by Natural Sciences and Engineering Research Council of Canada (www.nserc.ca) and Fonds de recherche du Québec ± Nature et technologies (www.frqnt.gouv.qc.ca)Versión del editor
https://doi.org/10.1371/journal.pone.0186058Materias
Wavelet Imaging on Multiple Scales (WIMS); Osteosarcoma cell; Disassembly or elongation; Mutant case; MedicinaDerechos
© 2017 Toplak et al.Resumen
We introduce and use Wavelet Imaging on Multiple Scales (WIMS) as an improvement to fluorescence correlation spectroscopy to measure physical processes and features that occur across multiple length scales. In this study, wavelet transforms of cell images are used to characterize molecular dynamics at the cellular and subcellular levels (i.e. focal adhesions). We show the usefulness of the technique by applying WIMS to an image time series of a migrating osteosarcoma cell expressing fluorescently labelled adhesion proteins, which allows us to characterize different components of the cell ranging from optical resolution scale through to focal adhesion and whole cell size scales. Using WIMS we measured focal adhesion numbers, orientation and cell boundary velocities for retraction and protrusion. We also determine the internal dynamics of individual focal adhesions undergoing assembly, disassembly or elongation. Thus confirming as previously shown, WIMS reveals that the number of adhesions and the area of the protruding region of the cell are strongly correlated, establishing a correlation between protrusion size and adhesion dynamics. We also apply this technique to characterize the behavior of adhesions, actin and myosin in Chinese hamster ovary cells expressing a mutant form of myosin IIB (1935D) that displays decreased filament stability and impairs front-back cell polarity. We find separate populations of actin and myosin at each adhesion pole for both the mutant and wild type form. However, we find these populations move rapidly inwards toward one another in the mutant case in contrast to the cells that express wild type myosin IIB where those populations remain stationary. Results obtained with these two systems demonstrate how WIMS has the potential to reveal novel correlations between chosen parameters that belong to different scales
Lista de ficheros
Google Scholar:Toplak, Tim
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Palmieri, Benoit
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Juanes-García, Alba
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Vicente-Manzanares, Miguel
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Grant, Martin
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Wiseman, Paul W.
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