Inhibition of the Maillard reaction by phytochemicals composing an aqueous coffee silverskin extract via a mixed mechanism of action
Entidad
UAM. Departamento de Química AgrícolaEditor
MDPI AGFecha de edición
2019-09-25Cita
10.3390/foods8100438
Foods 8.10 (2019): 438
ISSN
2304-8158DOI
10.3390/foods8100438Financiado por
M.R.H thanks the JAE Intro fellowship (JAEINT_15_00086) and the FPU predoctoral program of the Ministry of Science, Innovation, and Universities (FPU15/04238)Versión del editor
https://doi.org/10.3390/foods8100438Materias
Advanced glycation end products (AGE); Antioxidants; Chlorogenic acid; Coffee silverskin extract; Genistein; Maillard reaction; Melatonin; Protein glycation; Protein glycoxidation; Biología y Biomedicina / Biología; Ciencia y Tecnología de Alimentos; QuímicaDerechos
© 2019 by the authors. Licensee MDPI, Basel, SwitzerlandResumen
This work aimed to evaluate the contribution of isoflavones and melatonin to the aqueous extract obtained from the coffee silverskin (CSE) antiglycative properties, which has not been previously studied. To achieve this goal, two model systems constituted by bovine serum albumin (BSA) and reactive carbonyls (glucose or methylglyoxal) in the presence or absence of pure phytochemicals (chlorogenic acid (CGA), genistein, and melatonin) and CSE were employed. Glucose was used to evaluate the effect on the formation of glycation products formed mainly in the early stage of the reaction, while methylglyoxal was employed for looking at the formation of advanced products of the reaction, also called methylglyoxal-derivative advanced glycation end products (AGE) or glycoxidation products. CGA inhibited the formation of fructosamine, while genistein and melatonin inhibited the formation of advanced glycation end products and protein glycoxidation. It was also observed that phenolic compounds from CSE inhibited protein glycation and glycoxidation by forming BSA-phytochemical complexes. CSE showed a significant antiglycative effect (p < 0.05). Variations in the UV-Vis spectrum and the antioxidant capacity of protein fractions suggested the formation of protein-phytochemical complexes. Fluorescence quenching and in silico analysis supported the formation of antioxidant-protein complexes. For the first time, we illustrate that isoflavones and melatonin may contribute to the antiglycative/antiglycoxidative properties associated with CSE. CGA, isoflavones, and melatonin composing CSE seem to act simultaneously by different mechanisms of action
Lista de ficheros
Google Scholar:Rebollo-Hernanz, Miguel
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Fernández-Gómez, Beatriz
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Herrero, Miguel
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Aguilera Gutiérrez, Yolanda
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Martín Cabrejas, M. Ángeles
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Uribarri, Jaime
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Del Castillo, María Dolores
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