Dual imaging gold nanoplatforms for targeted radiotheranostics
Publisher
MDPI, Basel, SMwitzerlandDate
2020-01-22Citation
10.3390/ma13030513
Materials 13 (2020): 513
ISSN
1996-1944 (print)DOI
10.3390/ma13030513Funded by
This research was funded by FCT (Portuguese Foundation for Science and Technology), grant numbers EXCL/QEQ-MED/0233/2012, UID/Multi/04349/2013 and PTDC/MED-QUI/29649/2017. CFGCG and MMCAC thank FCT and FEDER through the COMPETE Program for funding the CQC (UID/QUI/00313/2013 and PEst-OE/QUI/UI0313/2014). P.L-L. thanks Ministry of Economy, Industry and Competitiviy for SAF2017-83043-R, and Comunity of Madrid, FEDER and FSE for S2017/BMD-3688Project
Gobierno de España. SAF2017-83043-R; Comunidad de Madrid. S2017/BMD-3688/MULTI-TARGETEditor's Version
http://doi.org/10.3390/ma13030513Subjects
Bombesin; Gold nanoparticles; MRI; Multimodality; PC3 tumor; Radiosensitization; SPECT; MedicinaRights
© 2020 by the authors.Abstract
Gold nanoparticles (AuNPs) are interesting for the design of new cancer theranostic tools, mainly due to their biocompatibility, easy molecular vectorization, and good biological half-life. Herein, we report a gold nanoparticle platform as a bimodal imaging probe, capable of coordinating Gd3+ for Magnetic Resonance Imaging (MRI) and 67Ga3+ for Single Photon Emission Computed Tomography (SPECT) imaging. Our AuNPs carry a bombesin analogue with anity towards the gastrin releasing peptide receptor (GRPr), overexpressed in a variety of human cancer cells, namely PC3 prostate cancer cells. The potential of these multimodal imaging nanoconstructs was thoroughly investigated by the assessment of their magnetic properties, in vitro cellular uptake, biodistribution, and radiosensitisation assays. The relaxometric properties predict a potential T1-and T2-MRI application. The promising in vitro cellular uptake of 67Ga/Gd-based bombesin containing particles was confirmed through biodistribution studies in tumor bearing mice, indicating their integrity and ability to target the GRPr. Radiosensitization studies revealed the therapeutic potential of the nanoparticles. Moreover, the DOTA chelating unit moiety versatility gives a high theranostic potential through the coordination of other therapeutically interesting radiometals. Altogether, our nanoparticles are interesting nanomaterial for theranostic application and as bimodal T1-and T2-MRI / SPECT imaging probes.
Files in this item
Google Scholar:Silva, Francisco
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Paulo, António
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Pallier, Agnès
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Même, Sandra
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Tóth, Éva
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Gano, Lurdes
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Marques, Fernanda
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Geraldes, Carlos F.G.C.
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Castro, M. Margarida C.A.
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Cardoso, Ana M.
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Jurado, Amália S.
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López-Larrubia, Pilar
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Lacerda, Sara
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Campello, Maria Paula Cabral
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