Amplification-free detection of SARS-CoV-2 using gold nanotriangles functionalized with oligonucleotides
Author
Del Caño Ochoa, Rafael; García Mendiola, Tania; García Nieto, Daniel; Álvaro, Raquel; Luna, Mónica; Alarcón Iniesta, Hernán; Coloma Ciudad, Rocío; Diaz, Ciro Rodríguez; Milán Rois, Paula; Castellanos, Milagros; Abreu, Melanie; Cantón, Rafael; Galán, Juan Carlos; Pineda, Teresa; Pariente Alonso, Félix; Miranda Soriano, Rodolfo; Somoza, Álvaro; Lorenzo Abad, EncarnaciónEntity
UAM. Departamento de Química Analítica y Análisis InstrumentalPublisher
SpringerDate
2022-04-01Citation
10.1007/s00604-022-05272-y
Microchimica Acta 189 (2022): 171
ISSN
0026-3672 (print); 1436-5073 (online)DOI
10.1007/s00604-022-05272-yFunded by
Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. This work is funded by the Community of Madrid (TRANSNANOAVANSENS S2018/NMT-4349), Spanish Ministry of Economy and Competitiveness (PID2020-116728RBI00, CTQ2015-71955-RED ELECTROBIONET), and the Community of Madrid (project S2018/NMT-4291 TEC2SPACE), Spanish Ministry of Economy and Competitiveness (project CSIC13-4E-1794) and EU (FEDER, FSE). RdC received grants from UAM, Banco Santander, Fundación IMDEA (convocatoria CRUE-CSIC-SANTANDER, fondo supera 2020, project with reference 10.01.03.02.41). JCG received grants from Ramón y Cajal Institute for Health Research (IRYCIS), Madrid, Spain (COVID-19 Grant, 2020/0154). This research was also funded by the Spanish Ministry of Economy and Competitiveness (SAF2017-87305-R, PID2020-119352RB-I00), Instituto de Salud Carlos III (FONDO-COVID19: COV20/00144 and COV20/00122). P.M.R received grants from the Ministry of Economy, Industry and competitiveness of Spain (FPI grant (BES-2017.082521)). IMDEA Nanociencia received grants from the “Severo Ochoa” Programme for Centres of Excellence in R&D (MINECO, Grant SEV-2016–0686, CEX2020-001039-S)Project
Gobierno de España. CTQ2015-71955-RED ELECTROBIONET; Gobierno de España. CSIC13-4E-1794; Gobierno de España. SAF2017-87305-R; Gobierno de España. PID2020-119352RB-I00Editor's Version
https://doi.org/10.1007/s00604-022-05272-ySubjects
Amplification-free detection; AuNTs; Biosensor; SARS-CoV-2; QuímicaRights
© The Author(s) 2022Abstract
Gold nanotriangles (AuNTs) functionalized with dithiolated oligonucleotides have been employed to develop an amplification-free electrochemical biosensor for SARS-CoV-2 in patient samples. Gold nanotriangles, prepared through a seed-mediated growth method and exhaustively characterized by different techniques, serve as an improved electrochemical platform and for DNA probe immobilization. Azure A is used as an electrochemical indicator of the hybridization event. The biosensor detects either single stranded DNA or RNA sequences of SARS-CoV-2 of different lengths, with a low detection limit of 22.2 fM. In addition, it allows to detect point mutations in SARS-CoV-2 genome with the aim to detect more infective SARS-CoV-2 variants such as Alpha, Beta, Gamma, Delta, and Omicron. Results obtained with the biosensor in nasopharyngeal swab samples from COVID-19 patients show the possibility to clearly discriminate between non-infected and infected patient samples as well as patient samples with different viral load. Furthermore, the results correlate well with those obtained by the gold standard technique RT-qPCR, with the advantage of avoiding the amplification process and the need of sophisticated equipment
Files in this item
Google Scholar:Del Caño Ochoa, Rafael
-
García Mendiola, Tania
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García Nieto, Daniel
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Álvaro, Raquel
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Luna, Mónica
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Alarcón Iniesta, Hernán
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Coloma Ciudad, Rocío
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Diaz, Ciro Rodríguez
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Milán Rois, Paula
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Castellanos, Milagros
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Abreu, Melanie
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Cantón, Rafael
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Galán, Juan Carlos
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Pineda, Teresa
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Pariente Alonso, Félix
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Miranda Soriano, Rodolfo
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Somoza, Álvaro
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Lorenzo Abad, Encarnación
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