Kinase PLK1 regulates the disassembly of the lateral elements and the assembly of the inner centromere during the diakinesis/metaphase I transition in male mouse meiosis
Entity
UAM. Departamento de BiologíaPublisher
Frontiers MediaDate
2023-01-13Citation
10.3389/fcell.2022.1069946
Frontiers in Cell and Developmental Biology 10.13 (2023): 1069946
ISSN
2296-634X (online)DOI
10.3389/fcell.2022.1069946Project
Gobierno de EspañaPID2020-117491GB-I00Editor's Version
https://doi.org/10.3389/fcell.2022.1069946Subjects
H2AT120ph; H3T3ph; PLK1; Inner Centromere; Lateral Elements; Meiosis; Mouse; Biología y Biomedicina / BiologíaRights
© 2023 Gómez, Viera, Moreno-Mármol, Berenguer, Guajardo-Grence, Tóth, Parra and SujaAbstract
PLK1 is a serine/threonine kinase with crucial roles during mitosis. However, its involvement during mammalian male meiosis remains largely unexplored. By inhibiting the kinase activity of PLK1 using BI 2536 on organotypic cultures of seminiferous tubules, we found that the disassembly of SYCP3 and HORMAD1 from the lateral elements of the synaptonemal complex during diakinesis is impeded. We also found that the normal recruitment of SYCP3 and HORMAD1 to the inner centromere in prometaphase I spermatocytes did not occur. Additionally, we analyzed the participation of PLK1 in the assembly of the inner centromere by studying its implication in the Bub1-H2AT120ph-dependent recruitment of shugoshin SGO2, and the Haspin-H3T3ph-dependent recruitment of Aurora B/C and Borealin. Our results indicated that both pathways are regulated by PLK1. Altogether, our results demonstrate that PLK1 is a master regulator of the late prophase I/metaphase I transition in mouse spermatocytes
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Google Scholar:Gómez Lencero, Rocío
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Viera Vicario, Alberto
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Moreno-Mármol, Tania
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Berenguer, Inés
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Guajardo-Grence, Andrea
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Tóth, Attila
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Parra Catalán, María Teresa
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Suja Sánchez, José Ángel
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