A targeted genetic screen identifies crucial players in the specification of the Drosophila abdominal Capaergic neurons
Entity
UAM. Departamento de BiologíaPublisher
Elsevier ScientificDate
2011Citation
10.1016/j.mod.2011.01.002
Mechanisms of Development 128.3-4 (2011): 208-221
ISSN
0925-4773DOI
10.1016/j.mod.2011.01.002Funded by
This work was supported by a grant from the Spanish Ministerio de Ciencia e Innovación (BFU-2008-04683-C02-02 to L.T.).Project
Gobierno de España. BFU-2008-04683-C02-02Editor's Version
http://dx.doi.org/10.1016/j.mod.2011.01.002Subjects
Drosophila; Temporal genes; Neuropeptidergic cell identity; CAPA; Progenitor neuroblasts; Biología y Biomedicina / BiologíaAbstract
The central nervous system contains a wide variety of neuronal subclasses generated by neural progenitors. The achievement of a unique neural fate is the consequence of a sequence of early and increasingly restricted regulatory events, which culminates in the expression of a specific genetic combinatorial code that confers individual characteristics to the differentiated cell. How the earlier regulatory events influence post-mitotic cell fate decisions is beginning to be understood in the Drosophila NB 5-6 lineage. However, it remains unknown to what extent these events operate in other lineages. To better understand this issue, we have used a very highly specific marker that identifies a small subset of abdominal cells expressing the Drosophila neuropeptide Capa: the ABCA neurons. Our data support the birth of the ABCA neurons from NB 5-3 in a cas temporal window in the abdominal segments A2–A4. Moreover, we show that the ABCA neuron has an ABCA-sibling cell which dies by apoptosis. Surprisingly, both cells are also generated in the abdominal segments A5–A7, although they undergo apoptosis before expressing Capa. In addition, we have performed a targeted genetic screen to identify players involved in ABCA specification. We have found that the ABCA fate requires zfh2, grain, Grunge and hedgehog genes. Finally, we show that the NB 5-3 generates other subtype of Capa-expressing cells (SECAs) in the third suboesophageal segment, which are born during a pdm/cas temporal window, and have different genetic requirements for their specification.
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Google Scholar:Gabilondo, Hugo
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Losada-Pérez, María
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Saz, Delia del
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Molina Balsa, Isabel
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León Álvarez, Yolanda
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Canal, Inmaculada
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Torroja Fungairiño, Laura
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Benito Sipos, Jonathan
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