Mañana, JUEVES, 24 DE ABRIL, el sistema se apagará debido a tareas habituales de mantenimiento a partir de las 9 de la mañana. Lamentamos las molestias.
Study on the 3-hydroxy-3-methyl-glutaryl CoA reductase inhibitory properties of Agaricus bisporus and extraction of bioactive fractions using pressurised solvent technologies
Entity
UAM. Departamento de Química Física AplicadaPublisher
Society of Chemical Industry; WilleyDate
2013-08-30Citation
10.1002/jsfa.6102
Journal of the Science of Food and Agriculture 93. 11(2013): 2789-2796
ISSN
0022-5142 (print); 1097-0010 (online)DOI
10.1002/jsfa.6102Funded by
The research was supported by the European Union within the 7th framework programme (7FM-PEOPLE-2009-IIF Project 251285), by the ALIBIRD-CM S2009/AGR-1469 and ADER 2010-I-ID-0093 regional programs from respectively the Community of Madrid and La Rioja (Spain), and by the AGL2010-21537 national R+D program from the Spanish Ministry of Science and Innovation.Project
Comunidad de Madrid. S2009/AGR-1469/ALIBIRD; info:eu-repo/grantAgreement/EC/FP7/251285Editor's Version
http://dx.doi.org/10.1002/jsfa.6102Subjects
HMGCoA reductase cholesterol; Pressurised solvent extraction (PSE); Supercritical fluid extraction (SFE); White button mushroom; Agaricus bisporus; Basidiomycota; Rattus; QuímicaRights
© 2013 Society of Chemical IndustryAbstract
BACKGROUND: Agaricus bisporus mushrooms were able to lower cholesterol levels in hypercholesterolaemic rats and it was suggested that dietary fibre might inhibit cholesterol absorption. However, A. bisporus extracts were also able to inhibit the 3-hydroxy-3-methyl-glutaryl CoA reductase (HMGCR, the key enzyme in the cholesterol biosynthetic pathway) and this might also contribute to the observed lowering of cholesterol levels in serum. RESULTS: The methanol-water extracts obtained from A. bisporus were able to inhibit up to 60% the HMGCR activity using an in vitro assay. The HMGCR inhibitory capacities depended on cultivation conditions, strains, etc. The potential inhibitors were not statins, they might be β-glucans able to scavenge the substrate and impair the enzymatic reaction. They were present during all mushroom developmental stages and similarly distributed through all the tissues including the parts discarded as a by-product. Accelerated solvent extractions using 1:1 ethanol-water as pressurised solvent (10.7 MPa, 25°C, five cycles of 5 min) were more effective in the extraction of the HMGCiR inhibitor(s) than supercritical fluid extractions (9 MPa, 40°C) using CO2 with 10% ethanol. CONCLUSION: A mushroom cultivation and two extraction procedures were optimised to obtain fractions from A. bisporus with high HMGCR inhibitory activities to design novel ingredients for hypocholesterolaemic functional foodstuffs
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Google Scholar:Gil Ramírez, Alicia
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Clavijo, Cristina
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Palanisamy, Marimuthu
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Ruiz Rodríguez, Alejandro
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Navarro-Rubio, María
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Pérez, Margarita
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Reglero Rada, Guillermo J.
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Soler Rivas, Cristina
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Marín, Francisco R.
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