Role of KLF6 as early predictor in human colorectal cancer | Papel de KLF6, como factor de predicción precoz en cáncer colorrectal humano

Abstract

Colorectal cancer (CRC) is the second most common type of cancer in the European population. Currently there molecular biomarkers that can be used for early detection of cancer of CRC. Is a tumor suppressor KLF6 associated with several types of cancers. Our hypothesis is that KLF6 can be an excellent marker for the early diagnosis of CRC. To study the implication of KLF6 in CRC, we selected 15 biopsies of each stage (T1, T2 and T3) from the archives of the Pathology Department of Defense Central Hospital Gómez-Ulla, which had areas of affected tissue (tumor) and unaffected areas (non-tumor). This study was performed histological, immunohistochemical and RT-PCR, based on the expression of three genes, markers Ki67 and p53 as positive and as a marker in KLF6 study. The results showed that the expression of KLF6 is directly related to increased malignancy cell adenocarcinomas, corroborated by RT-PCR, showing the gradual emergence of alternative forms processing, corresponding to no KLF6. This protein was expressed both cytoplasmic and nuclear in the early stages T1 and T2, disappearing at the nuclear level in the most advanced stage (T3). KLF6 conclude that a good CRC tumor marker because it shows patterns of expression level increased cytoplasmic and nuclear level decreasing