|dc.contributor.advisor||Sánchez Cabrero, Benigno||
|dc.contributor.author||Sánchez Muñoz, Marta||
|dc.contributor.other||UAM. Departamento de Biología Molecular||es_ES
|dc.contributor.other||Centro de Investigaciones Energéticas, Medioambientales y Tecnológicas (CIEMAT)||es_ES
|dc.contributor.other||Centro de Biología Molecular Severo Ochoa (CBM)||es_ES
|dc.description||Tesis doctoral inédita leída en la Universidad Autónoma de Madrid, Facultad de Ciencias, Departamento de Biología Molecular. Fecha de lectura: 29-07-2013||es_ES
|dc.description.abstract||Nowadays, people in developed countries spent most of their time in different indoor
environments and the concern about indoor air quality has increased at the same time as the
illnesses related with unhealthy buildings. Indoor Air Quality (IAQ) can be significantly
deteriorated by high levels of bioaerosols that may cause infections, toxic reactions or
allergies in building occupants. Indeed, there is no standard method for the quantification of
this kind of pollution and several protocols and sampling devices are used. Consequently, there
is an increasing demand for a systematic control of the indoor bioaerosols and efficient air
In this study, several methods for air sampling were tested such as gravitational settling
on agar surfaces (sedimentation plates) or buffers (PBS), filtration or inertial impaction
samplers. Moreover, three commonly used portable air samplers were compared: DUO SAS
SUPER 360, SAMPL’AIR and SPIN AIR units and tested simultaneously for bacteria
quantification in a laboratory room in realistic conditions.
A fungal and bacterial characterization was done in buildings with different geographical
location (Almería-Madrid-Soria), different age and at different seasons of the year.
Bacterial and fungal samples were incubated and colony-forming units were counted.
Polymerase Chain Reaction (PCR) technique (16S ARNr amplification) and subsequent
sequenciation were used for bacterial species identification. For fungal identification microand
macro-morphology examination of the colonies were used. Additionally, several samples
isolated from Madrid building were identified by the amplification of the Internal
Transcribed Spacer (ITS) region. Pathogenic bacteria, such as Pseudomonas aeruginosa,
Escherichia coli and Francisella tularensis were isolated in most of the sampled buildings.
Fungal identification results showed different species of Cladosporium, Alternaria, Penicillium,
Aspergillus and an important number of yeasts in all the indoor environments sampled. These
fungal genera can cause asthma, allergies or a contribution to the development of the Sick
Building Syndrome (SBS).
A new approach based on heterogeneous photocatalysis was tested to treat real indoor air.
An annular UV-photocatalytic reactor was developed at laboratory scale where real indoor air
was treated without recirculation. Results showed an efficient removal of airborne bacteria
using TiO2 photocatalyst supported on transparent polymeric monoliths irradiated with UVA.
As the laboratory scale photocatalytic tests were promising, a second photoreactor was
designed, constructed and installed in an office. This demonstration scale photoreactor was
attached to an HVAC system and was able to treat the indoor air by both photocatalysis or
photolysis. For photocatalytic treatments aluminum monoliths were coated with a TiO2 sol-gel.
The photoreactor was efficient to remove 97-99% of airborne bacteria when the catalyst
was illuminated with three lamps during four hours and 81% of airborne fungi when nine lamps
were lit inside the reactor.
The experiments performed proved that this technology was efficient for bacteria and
fungi removal and could compete with the dangerous conventional photolytic treatment with
|dc.subject.other||Aire - Contaminación interior - Tesis doctorales||es_ES
|dc.subject.other||Hongos patógenos - Tesis doctorales||es_ES
|dc.subject.other||Bacterias - Tesis doctorales||es_ES
|dc.subject.other||Fotocatalisis - Tesis doctorales||es_ES
|dc.title||Caracterización y tratamiento fotocatalítico de hongos y bacterias de aire interior||es_ES
|dc.subject.eciencia||Biología y Biomedicina / Biología||es_ES
|dc.rights.cc||Reconocimiento – NoComercial – SinObraDerivada||es_ES
|dc.facultadUAM||Facultad de Ciencias||