INF2 promotes the formation of detyrosinated microtubules necessary for centrosome reorientation in T cells
Entity
UAM. Departamento de Biología MolecularPublisher
Elsevier B.V.Date
2012-09-17Citation
10.1083/jcb.201202137
Journal of Cell Biology 198. 6 (2012): 1025-1037
ISSN
0021-9525 (print); 1540-8140 (online)DOI
10.1083/jcb.201202137Funded by
This work was supported by grants BFU2009-07886 and CONSOLIDER COAT CSD2009-00016 to M.A. Alonso, and BFU2011-22859 to I. Correas (all of them from the Ministerio de Economía y Competitividad, Spain), and grant S2010/BMD-2305 from the Comunidad de Madrid to I. CorreasProject
Comunidad de Madrid. S2010/BMD-2305/CS INTERACTOMICSEditor's Version
http://dx.doi.org/10.1083/jcb.201202137Subjects
Biología y Biomedicina / BiologíaRights
© 2012 Andrés-Delgado et al.Abstract
T cell antigen receptor-proximal signaling components, Rho-family GTPases, and formin proteins DIA1 and FMNL1 have been implicated in centrosome reorientation to the immunological synapse of T lymphocytes. However, the role of these molecules in the reorientation process is not yet defined. Here we find that a subset of microtubules became rapidly stabilized and that their α-tubulin subunit posttranslationally detyrosinated after engagement of the T cell receptor. Formation of stabilized, detyrosinated microtubules required the formin INF2, which was also found to be essential for centrosome reorientation, but it occurred independently of T cell receptor-induced massive tyrosine phosphorylation. The FH2 domain, which was mapped as the INF2 region involved in centrosome repositioning, was able to mediate the formation of stable, detyrosinated microtubules and to restore centrosome translocation in DIA1-, FMNL1-, Rac1-, and Cdc42-deficient cells. Further experiments indicated that microtubule stabilization was required for centrosome polarization. Our work identifies INF2 and stable, detyrosinated microtubules as central players in centrosome reorientation in T cells
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Google Scholar:Andrés Delgado, Laura
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Antón, Olga M.
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Bartolini, Francesca
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Ruiz-Sáenz, Ana
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Correas Hornero, María Isabel
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Gundersen, Gregg G.
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Alonso, Miguel Angel
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