Copper microlocalisation, ultrastructural alterations and antioxidant responses in the nodules of white lupin and soybean plants grown under conditions of copper excess
Entity
UAM. Departamento de Química AgrícolaPublisher
Elsevier B.V.Date
2012-12-01Citation
10.1016/j.envexpbot.2012.04.017
Environmental and Experimental Botany 84 (2012): 52-60
ISSN
0098-8472 (print)DOI
10.1016/j.envexpbot.2012.04.017Funded by
Funding for this study was provided by the Spanish MCyT (project CTM2010-21922-C02-02/TECNO), the Autonomous Community of Madrid (project S2009/AMB-1478) and the Junta de Comunidades de Castilla-La Mancha (project POII10-0211-5015)Project
Comunidad de Madrid. S2009/AMB-1478/EIADESEditor's Version
http://dx.doi.org/10.1016/j.envexpbot.2012.04.017Subjects
Copper excess; Energy-dispersive X-ray microanalysis; Glycine max L.; Lupinus albus L.; Nodule ultrastructure; Oxidative stress; QuímicaNote
NOTICE: this is the author’s version of a work that was accepted for publication in Environmental and Experimental Botany. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Environmental and Experimental Botany 84 (2012): 52-60 DOI 10.1016/j.envexpbot.2012.04.017Rights
© 2012 ElsevierEsta obra está bajo una licencia de Creative Commons Reconocimiento-NoComercial-SinObraDerivada 4.0 Internacional.
Abstract
Copper (Cu) is a heavy metal which, at high concentrations, is toxic to organisms. Some plants, however, possess systems for dealing with excess Cu, such as its cell localisation, and have antioxidant enzymes that help to reduce the oxidative stress. The present work examines the microlocalisation of Cu and the antioxidant enzyme activity present in the nodules of white lupin and soybean plants grown hydroponically for 35 days in the presence of 1.6μM (control) or 192μM (excess) Cu. The effect of these conditions on nodule (ultra)structure was also examined. Energy-dispersive X-ray microanalysis showed the cell walls to be the main area of Cu binding in the inner and outer cortex and infected zone of white lupin nodules grown under the excess Cu conditions, while in soybean a high Cu signal was detected inside cells (cytoplasm or vacuoles) both in the inner cortex and infected zone. At the tissue level, an increasing Cu gradient was seen from the outer towards the inner nodule cortex in white lupin nodules, while the opposite was seen in soybean. Cu excess also induced oxidative stress and promoted damage to the ultrastructure of nodules. In the white lupin infected cells, a breakdown of the peribacteroidal membrane was seen, along with an increased number of vesicles in the cytosol of these cells. In the infected cells of the soybean nodules, the bacteroidal membrane became degraded and precipitation was seen within the vacuoles of the infected and uninfected cells. Finally, the white lupin nodules seemed to be more sensitive to Cu excess than those of soybean, with the nodulation process, N2 fixation, and the ultrastructure of bacteroids more strongly affected. A less effective antioxidative stress response against Cu was also seen in white lupin than in soybean nodules: the excess copper treatment induced a smaller increase in the total thiol content and ascorbate peroxidase activity in white lupin nodules than in soybean nodules, and promoted a greater reduction in catalase activity
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Google Scholar:Sánchez-Pardo, Beatriz
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Fernández-Pascual, Mercedes M.
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Zornoza, Pilar
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