Mitigation of Cu stress by legume-Rhizobium symbiosis in white lupin and soybean plants
EntityUAM. Departamento de Química Agrícola
10.1016/j.ecoenv.2014.01.016Ecotoxicology and Environmental Safety 102.1 (2014): 1-5
ISSN1090-2414 (on line); 0147-6513 (print)
Funded byFunding for this study was provided by the Spanish Ministry of Science and Technology (project CTM2010-21922-C02-02/TECNO), the Spanish Ministry of the Economy and Competitiveness (AGL2012-39715-C03-03/MINECO), and the Comunidad de Madrid (project S2009/AMB-1478)
ProjectComunidad de Madrid. S2009/AMB-1478/EIADES
SubjectsCopper excess; Glycine max L.; Legume-Rhizobium symbiosis; Lupinus albus L.; Nitrogen fixation; Phytoextraction; Química
NoteNOTICE: this is the author’s version of a work that was accepted for publication in Ecotoxicology and Environmental Safety. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Ecotoxicology and Environmental Safety, 102,1, (2014) DOI http://dx.doi.org/10.1016/j.ecoenv.2014.01.016
Rights© 2014 Elsevier inc.
Esta obra está bajo una licencia de Creative Commons Reconocimiento-NoComercial-SinObraDerivada 4.0 Internacional.
The effect of Bradyrhizobium-legume symbiosis on plant growth, toxicological variables and Cu bioaccumulation was studied in white lupin and soybean plants treated with 1.6, 48, 96 and 192μM Cu. In both species, those plants grown in the presence of root nodule-forming symbiotic Bradyrhizobium showed less root and shoot growth reduction, plus greater translocation of Cu to the shoot, than those grown without symbiotic Bradyrhizobium. The effective added concentrations of Cu that reduced shoot and root dry weight by 50% (EC50), and the critical toxic concentration that caused a 10% reduction in plant growth (CTC10%), were higher in plants grown with symbiotic Bradyrhizobium, and were in general higher in the roots whether the plants were grown with or without these bacteria. The production of malondialdehyde and total thiols was stimulated by Cu excess in the shoots and roots of white lupin grown with or without symbiotic Bradyrhizobium, but mainly in those without the symbionts. In contrast, in soybean, the increases in malondialdehyde and total thiols associated with rising Cu concentration were a little higher (1.2-5.0 and 1.0-1.6 times respectively) in plants grown with symbiotic Bradyrhizobium than without. Finally, the organ most sensitive to Cu excess was generally the shoot, both in white lupin and soybean grown with or without symbiotic Bradyrhizobium. Further, Bradyrhizobium-legume symbiosis appears to increase the tolerance to Cu excess in both legumes, but mainly in white lupin; plant growth was less reduced and CTC10% and EC50 values increased compared to plants grown without symbiotic Bradyrhizobium. Bradyrhizobium N2 fixation in both legumes would therefore seem to increase the phytoremediation potential of these plants when growing on Cu-contaminated sites
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