Cadmium in white lupin nodules: Impact on nitrogen and carbon metabolism
EntityUAM. Departamento de Química Agrícola
10.1016/j.jplph.2012.10.001Journal of Plant Physiology 170.3 (2013): 265-271
ISSN0176-1617 (print); 1618-1328 (on line)
Funded byFunding was provided by the Spanish MCyT (project CTM2010-21922-C02-02) and by the Autonomous Community of Madrid (project S2009/AMB-1478)
ProjectComunidad de Madrid. S2009/AMB-1478/EIADES; Gobierno de España. CTM2010-21922-C02-02
SubjectsCadmium microlocalisation; Carbohydrate metabolism; Energy-dispersive X-ray microanalysis; Lupinus albus L.; Nitrogen fixation; Química
NoteThis is the author’s version of a work that was accepted for publication in Journal of Plant Physiology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Plant Physiology, 170, 3, (2013) DOI http://dx.doi.org/10.1016/j.jplph.2012.10.001
Rights© 2012 Elsevier GmbH
Esta obra está bajo una licencia de Creative Commons Reconocimiento-NoComercial-SinObraDerivada 4.0 Internacional.
The aims of this work were to investigate the microlocalisation of cadmium (Cd) in Lupinus albus L. cv. Multolupa nodules, and to determine its effects on carbon and nitrogen metabolism. Nodulated white lupin plants were grown in a growth chamber with or without Cd (150μM). Energy-dispersive X-ray microanalysis showed the walls of the outer nodule cortex cells to be the main area of Cd retention, helping to reduce the harmful effect Cd might have on the amount of N2 fixed by the bacteroids. Sucrose synthase activity declined by 33% in the nodules of the Cd-treated plants, and smaller reductions were recorded in glutamine synthetase, aspartate aminotransferase, alkaline invertase and NADP-dependent isocitrate dehydrogenase activities. The Cd treatment also sharply reduced nodule concentrations of malate, succinate and citrate, while that of starch doubled, but that of sucrose experienced no significant change. In summary, the present results show that white lupins accumulate significant amounts of Cd in their root nodules. However, the activity of some enzymes involved in ammonium assimilation did decline, promoting a reduction in the plant N content. The downregulation of sucrose synthase limits the availability of carbon to the bacteroids, which might interfere with their respiration. Carbon metabolism therefore plays a primary role in the impaired function of the white lupin root nodule caused by Cd, while N metabolism appears to have a more secondary involvement
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