Nanostructured porous silicon micropatterns as a tool for substrate-conditioned cell research
EntityUAM. Departamento de Física Aplicada
PublisherSpringer New York
10.1186/1556-276X-7-396Nanoscale Research Letters 7(2011): 1-7
ISSN1931-7573 (print); 1556-276X (online)
Funded byThe authors gratefully acknowledge the financial support from MICINN under research project MAT2008-06858-C02-01/NAN and Comunidad de Madrid (Spain) under Project Microseres. Technical support from L García Pelayo is greatly appreciated
ProjectComunidad de Madrid. S2009/TIC-1476/MICROSERES
SubjectsCell adhesion; Cell guide; Human mesenchymal stem cells; Ion beam; Micropattern; Nanostructured porous silicon; Photoluminescence; Física
Rights© 2012 Punzón-Quijorna et al.
Esta obra está bajo una Licencia Creative Commons Atribución 4.0 Internacional.
The localized irradiation of Si allows a precise patterning at the microscale of nanostructured materials such as porous silicon (PS). PS patterns with precisely defined geometries can be fabricated using ion stopping masks. The nanoscale textured micropatterns were used to explore their influence as microenvironments for human mesenchymal stem cells (hMSCs). In fact, the change of photoluminescence emission from PS upon aging in physiological solution suggests the intense formation of silanol surface groups, which may play a relevant role in ulterior cell adhesion. The experimental results show that hMSCs are sensitive to the surface micropatterns. In this regard, preliminary β-catenin labeling studies reveal the formation of cell to cell interaction structures, while microtubule orientation is strongly influenced by the selective adhesion conditions. Relevantly, Ki-67 assays support a proliferative state of hMSCs on such nanostructured micropatterns comparable to that of standard cell culture platforms, which reinforce the candidature of porous silicon micropatterns to become a conditioning structure for in vitro culture of hMSCs
Google Scholar:Punzón Quijorna, Esther - Sánchez-Vaquero, Vanessa - Muñoz-Noval, Álvaro - Pérez-Roldán, Maria Jesús - Martín Palma, Raúl José - Rossi, François - Climent Font, Aurelio - Manso-Silván, Miguel - García-Ruíz, Josefa Predestinación - Torres-Costa, Vicente
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Conditioned bio-interfaces of silicon/porous silicon micro-patterns lead to the chondrogenesis of hMSCs Alarcón, H.; Ynsa, M. D.; Dang, Z. Y.; Torres-Costa, Vicente; Manso-Silván, M.; Wu, J. F.; Breese, M. B H; García-Ruiz, J. P.