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A novel melanocortin-4 receptor mutation MC4R-P272L associated with severe obesity has increased propensity to be ubiquitinated in the ER in the face of correct folding
dc.contributor.author | Granell, Susana | |
dc.contributor.author | Serra-Juhé, Clara | |
dc.contributor.author | Martos Moreno, Gabriel Ángel | |
dc.contributor.author | Díaz, Francisca | |
dc.contributor.author | Pérez-Jurado, Luis A. | |
dc.contributor.author | Baldini, Giulia | |
dc.contributor.author | Argente Oliver, Jesús | |
dc.contributor.other | UAM. Departamento de Pediatría | es_ES |
dc.date.accessioned | 2015-04-21T15:27:18Z | |
dc.date.available | 2015-04-21T15:27:18Z | |
dc.date.issued | 2012-12-12 | |
dc.identifier.citation | Plos One 7.12 (2012): e50894 | en_US |
dc.identifier.issn | 1932-6203 (online) | en_US |
dc.identifier.uri | http://hdl.handle.net/10486/665344 | |
dc.description.abstract | Heterozygous mutations in the melanocortin-4 receptor (MC4R) gene represent the most frequent cause of monogenic obesity in humans. MC4R mutation analysis in a cohort of 77 children with morbid obesity identified previously unreported heterozygous mutations (P272L, N74I) in two patients inherited from their obese mothers. A rare polymorphism (I251L, allelic frequency: 1/100) reported to protect against obesity was found in another obese patient. When expressed in neuronal cells, the cell surface abundance of wild-type MC4R and of the N74I and I251L variants and the cAMP generated by these receptors in response to exposure to the agonist, α-MSH, were not different. Conversely, MC4R P272L was retained in the endoplasmic reticulum and had reduced cell surface expression and signaling (by ≈3-fold). The chemical chaperone PBA, which promotes protein folding of wild-type MC4R, had minimal effects on the distribution and signaling of the P272L variant. In contrast, incubation with UBE-41, a specific inhibitor of ubiquitin activating enzyme E1, inhibited ubiquitination of MC4R P272L and increased its cell surface expression and signaling to similar levels as wild-type MC4R. UBE41 had much less profound effects on MC4R I316S, another obesity-linked MC4R variant trapped in the ER. These data suggest that P272L is retained in the ER by a propensity to be ubiquitinated in the face of correct folding, which is only minimally shared by MC4R I316S. Thus, studies that combine clinical screening of obese patients and investigation of the functional defects of the obesity-linked MC4R variants can identify specific ways to correct these defects and are the first steps towards personalized medicine | en_US |
dc.description.sponsorship | This work has been funded by Fondo de Investigación Sanitaria (PI09/91060, PI10/02512, PI01/00747), CIBERobn Instituto de Salud Carlos III (ISCIII), Fundación Mutua Madrileña (AP2561/2008), Fundación Endocrinología y Nutrición, the National Institutes of Health (R01DK080424 to GB), and the Arkansas Tobacco Settlement (to GB). CS-J and GAM-M were recipients of fellowships from ISCIII (FI08/00365 and CM05/00100, respectively) | es_ES |
dc.format.extent | 7 pag. | es_ES |
dc.format.mimetype | application/pdf | en |
dc.language.iso | eng | en |
dc.publisher | Public Library of Science | en_US |
dc.relation.ispartof | Plos One | en_US |
dc.rights | © 2012 Granell et al. | es_ES |
dc.subject.other | Alleles | en_US |
dc.subject.other | Child | en_US |
dc.subject.other | Cell Line | en_US |
dc.subject.other | Obesity | en_US |
dc.subject.other | Ubiquitin | en_US |
dc.subject.other | Protein Folding | en_US |
dc.title | A novel melanocortin-4 receptor mutation MC4R-P272L associated with severe obesity has increased propensity to be ubiquitinated in the ER in the face of correct folding | en_US |
dc.type | article | en_US |
dc.subject.eciencia | Medicina | es_ES |
dc.identifier.doi | 10.1371/journal.pone.0050894 | es_ES |
dc.identifier.publicationfirstpage | e50894 | es_ES |
dc.identifier.publicationissue | 12 | es_ES |
dc.identifier.publicationlastpage | e50894 | es_ES |
dc.identifier.publicationvolume | 7 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | en |
dc.rights.cc | Reconocimiento | es_ES |
dc.rights.accessRights | openAccess | en |
dc.authorUAM | Argente Oliver, Jesús (100008) | |
dc.authorUAM | Martos Moreno, Gabriel Ángel (101491) | |
dc.facultadUAM | Facultad de Medicina | |
dc.institutoUAM | Instituto de Investigación Sanitaria Hospital Universitario de La Princesa (IIS-Princesa) |